{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:25:40Z","timestamp":1760865940661,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T00:00:00Z","timestamp":1441152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cucurbitacin B (CuB), one of the most abundant forms of cucurbitacins, is a promising natural anticancer drug candidate. Although the anticancer activity of CuB has been well demonstrated, information regarding the pharmacokinetics is limited. A rapid, selective and sensitive UPLC\u2010MS\/MS for CuB was developed and validated using hemslecin A (HeA) as internal standard (IS). Plasma samples were pre\u2010treated by liquid\u2013liquid extraction with dichloromethane. Separation was achieved on a reversed\u2010phase C<jats:sub>18<\/jats:sub> column (50 \u00d7 4.6 mm, 5 \u00b5m) at 35\u00b0C using isocratic elution with water\u2013methanol (25:75, v\/v) at a flow rate of 0.3 mL\/min. The analytes were monitored by a triple quadrupole tandem mass spectrometer with positive electrospray ionization mode. The calibration curve was linear (<jats:italic>r<\/jats:italic> &gt; 0.995) in a concentration range of 0.3\u2013100 ng\/mL with a limit of quantification of 0.3 ng\/mL. Intra\u2010 and inter\u2010day accuracy and precision were validated by percentage relative error and relative standard deviation, respectively, which were both lower than the limit of 15%. This assay was successfully applied to a pharmacokinetic study of CuB in Wistar rats. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3571","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T11:49:18Z","timestamp":1437738558000},"page":"503-507","source":"Crossref","is-referenced-by-count":10,"title":["Development and validation of a UPLC\u2010MS\/MS method for the determination of cucurbitacin B in rat plasma and application to a pharmacokinetic study"],"prefix":"10.1002","volume":"30","author":[{"given":"Waiou","family":"Zhao","sequence":"first","affiliation":[{"name":"Cardiology Department the First Hospital of Jilin University  Changchun 130021 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dahai","family":"Xu","sequence":"additional","affiliation":[{"name":"Emergency Department the First Hospital of Jilin University  Changchun 130021 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