{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:00:44Z","timestamp":1760061644464,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,9,18]],"date-time":"2015-09-18T00:00:00Z","timestamp":1442534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Plasmodium falciparum<\/jats:italic> (<jats:italic>Pf<\/jats:italic>) like most other organisms, has a sophisticated antioxidant system, part of which includes glutathione reductase (GR). GR works by recycling toxic glutathione disulfide to glutathione, thereby reducing reactive oxygen species and making a form of glutathione (GSH) the parasite can use. Inhibition of this enzyme in <jats:italic>Pf<\/jats:italic> impedes parasite growth. In addition, it has been confirmed that <jats:italic>Pf<\/jats:italic>GR is not identical to human GR. Thus, <jats:italic>Pf<\/jats:italic>GR is an excellent target for antimalarial drug development. A functional assay utilizing liquid chromatography\u2013mass spectrometry was developed to specifically identify and evaluate inhibitors of <jats:italic>Pf<\/jats:italic>GR. Using recombinant <jats:italic>Pf<\/jats:italic>GR enzyme and 1,4\u2010naphthoquinone (1) as a reference compound and 4\u2010nitrobenzothiadiazole (2) and methylene blue (3) as additional compounds, we quantified the concentration of GSH produced compared with a control to determine the inhibitory effect of these compounds. Our results coincide with that presented in literature: compounds 1\u20133 inhibit <jats:italic>Pf<\/jats:italic>GR with IC<jats:sub>50<\/jats:sub> values of 2.71, 8.38, and 19.23\u2009\u00b5<jats:sc>m<\/jats:sc>, respectively. Good precision for this assay was exhibited by low values of intraday and interday coefficient of variation (3.1 and 2.4%, respectively). Thus, this assay can be used to screen for other potential inhibitors of <jats:italic>Pf<\/jats:italic>GR quickly and accurately. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3580","type":"journal-article","created":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T10:10:07Z","timestamp":1439028607000},"page":"543-547","source":"Crossref","is-referenced-by-count":3,"title":["Development of a functional assay to detect inhibitors of <i>Plasmodium falciparum<\/i> glutathione reductase utilizing liquid chromatography\u2013mass spectrometry"],"prefix":"10.1002","volume":"30","author":[{"given":"Lexi","family":"Burkard","sequence":"first","affiliation":[{"name":"Department of Drug Discovery and Development Harrison School of Pharmacy  4306 Walker Building, Auburn University Auburn AL 36849 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Scheuermann","sequence":"additional","affiliation":[{"name":"Department of Drug Discovery and Development Harrison School of Pharmacy  4306 Walker Building, Auburn University Auburn AL 36849 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johayra","family":"Simithy","sequence":"additional","affiliation":[{"name":"Department of Drug Discovery and Development Harrison School of Pharmacy  4306 Walker Building, Auburn University Auburn AL 36849 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela I.","family":"Calder\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Drug Discovery and Development Harrison School of Pharmacy  4306 Walker Building, Auburn University Auburn AL 36849 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.07.011"},{"key":"e_1_2_7_3_1","first-page":"92","article-title":"Glutathione metabolism in parasitic protozoa","volume":"31","author":"Banyal HS","year":"2007","journal-title":"Journal of Parasitic Diseases"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007695200"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.123323"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-6577-3"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tpb.2008.10.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm010268g"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2007.08.004"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2008.03.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm7009292"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20019"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(05)03620-2"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00345.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/b802649c"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04257.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja201729z"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.4269\/ajtmh.77.6.suppl.170","article-title":"Intensity of malaria transmission and the spread of Plasmodium falciparum\u2010resistant malaria: a review of epidemiologic field evidence","volume":"77","author":"Talisuna AO","year":"2007","journal-title":"American Journal of Tropical Medicine Hygiene"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3776"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3580","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3580","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:53:57Z","timestamp":1760039637000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3580"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,18]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1002\/bmc.3580"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3580","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2015,9,18]]}}}