{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:40:53Z","timestamp":1760046053783,"version":"build-2065373602"},"reference-count":5,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2004,5,5]],"date-time":"2004-05-05T00:00:00Z","timestamp":1083715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A speci\ufb01c, accurate, precise and reproducible high\u2010performance liquid chromatography (HPLC) method was developed for the estimation of DRF\u20104367, a novel cyclooxygenase\u20102 inhibitor in rat plasma. The assay procedure involved simple liquid\/liquid extraction of DRF\u20104367 and internal standard (IS, celecoxib) from plasma into dichloromethane. The organic layer was separated and evaporated under a gentle stream of nitrogen at 40\u00b0C. The residue was reconstituted in the mobile phase and injected onto a Kromasil KR 100\u20105C<jats:sub>18<\/jats:sub> column (4.6 \u00d7 250 mm, 5 \u00b5m). The mobile phase consisting of 0.01 <jats:sc>m<\/jats:sc> potassium dihydrogen <jats:italic>ortho<\/jats:italic>\u2010phosphate (pH 3.2) and acetonitrile (40:60, v\/v) was used at a \ufb02ow rate of 1.0 mL\/min. The eluate was monitored using an UV detector set at 247 nm. The ratio of peak area of analyte to IS was used for quanti\ufb01cation of plasma samples. Nominal retention times of DRF\u20104367 and IS were 6.6 and 11.2 min, respectively. The standard curve for DRF\u20104367 was linear (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.999) in the concentration range 0.1\u201320 \u00b5g\/mL. Absolute recovery was &gt;86% from rat plasma for both analyte and IS. The lower limit of quanti\ufb01cation of DRF\u20104367 was 0.1 \u00b5g\/mL. The inter\u2010 and intra\u2010day precisions in the measurement of quality control samples, 0.1, 0.3, 8.0 and 15.0 \u00b5g\/mL, were in the range 6.93\u20139.34% relative standard deviation (RSD) and 0.48\u20136.59% RSD, respectively. Accuracy in the measurement of QC samples was in the range 91.24\u2013109.36% of the nominal values. Analyte and IS were stable in the battery of stability studies, viz. benchtop, autosampler and freeze\u2013thaw cycles. Stability of DRF\u20104367 was established for 1 month at \u221280\u00b0C. The application of the assay to a pharmacokinetic study in rats is described. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.359","type":"journal-article","created":{"date-parts":[[2004,5,5]],"date-time":"2004-05-05T13:07:36Z","timestamp":1083762456000},"page":"576-580","source":"Crossref","is-referenced-by-count":2,"title":["HPLC method for determination of DRF\u20104367 in rat plasma: validation and its application to pharmacokinetics in Wistar rats"],"prefix":"10.1002","volume":"18","author":[{"given":"Ramesh","family":"Mullangi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raja Reddy","family":"Kallem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravi Kanth","family":"Bhamidipati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rao N. V. S.","family":"Mamidi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuggehally R.","family":"Srinivas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1201\/b14095","volume-title":"Pharmacokinetics","author":"Gibaldi M","year":"1982"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03190502"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600810324"},{"first-page":"1982","volume-title":"United States Pharmacopoeia","year":"1995","key":"e_1_2_1_5_1"},{"key":"e_1_2_1_6_1","unstructured":"SinghSK SaibabaV Ganapati ReddyP Srinivasa RaoK LohrayBB Seshagiri RaoC ParimalMisra Srinivasa RajuD RaoNVSM RameshM RavikanthB RajagopalanR VenkateswarluAandKoteswar RaoY.Journal of Medicinal Chemistry2003(Submitted)."}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.359","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:58:55Z","timestamp":1760043535000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5,5]]},"references-count":5,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1002\/bmc.359"],"URL":"https:\/\/doi.org\/10.1002\/bmc.359","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2004,5,5]]}}}