{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:53:02Z","timestamp":1777513982299,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A molecularly imprinted polymer (MIP) was prepared using (\u2212)\u2010norephedrine as the template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross\u2010linker and chloroform as the porogen. The MIP was used as a selective sorbent in the molecularly imprinted solid\u2010phase extraction (MIP\u2010SPE) of the psychoactive phenylpropylamino alkaloids, norephedrine and its analogs, cathinone and cathine, from Khat (<jats:italic>Catha edulis<\/jats:italic> Vahl. Endl.) leaf extracts prior to HPLC\u2010DAD analysis. The MIP was able to selectively extract the alkaloids from the aqueous extracts of Khat. Loading, washing and elution of the alkaloids bound to the MIP were evaluated under different conditions. The clean baseline of the Khat extract obtained after MIP\u2010SPE confirmed that a selective and efficient sample clean\u2010up was achieved. Good recoveries (90.0\u2013107%) and precision (RSDs 2.3\u20133.2%) were obtained in the validation of the MIP\u2010SPE\u2010HPLC procedure. The content of the three alkaloids in Khat samples determined after treatment with MIP\u2010SPE and a commercial Isolute C<jats:sub>18<\/jats:sub> (EC) SPE cartridge were in good agreement. These findings indicate that MIP\u2010SPE is a reliable method that can be used for sample pre\u2010treatment for the determination of Khat alkaloids in plant extracts or similar matrices and could be applicable in pharmaceutical, forensic and biomedical laboratories. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3643","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T21:29:07Z","timestamp":1446586147000},"page":"1007-1015","source":"Crossref","is-referenced-by-count":13,"title":["A (\u2212)\u2010norephedrine\u2010based molecularly imprinted polymer for the solid\u2010phase extraction of psychoactive phenylpropylamino alkaloids from Khat (<i>Catha edulis<\/i> Vahl. Endl.) chewing leaves"],"prefix":"10.1002","volume":"30","author":[{"given":"Minaleshewa","family":"Atlabachew","sequence":"first","affiliation":[{"name":"Tshwane University of Technology Department of Chemistry  Pretoria South Africa"},{"name":"Bahir Dar University Department of Chemistry  Bahir Dar Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson","family":"Torto","sequence":"additional","affiliation":[{"name":"Rhodes University Department of Chemistry  Grahamstown South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhagwan Singh","family":"Chandravanshi","sequence":"additional","affiliation":[{"name":"Addis Ababa University, College of Natural Sciences Department of Chemistry  Addis Ababa Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mesfin","family":"Redi\u2010Abshiro","sequence":"additional","affiliation":[{"name":"Addis Ababa University, College of Natural Sciences Department of Chemistry  Addis Ababa Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Chigome","sequence":"additional","affiliation":[{"name":"Rhodes University Department of Chemistry  Grahamstown South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kediemetse","family":"Mothibedi","sequence":"additional","affiliation":[{"name":"Rhodes University Department of Chemistry  Grahamstown South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Combrinck","sequence":"additional","affiliation":[{"name":"Tshwane University of Technology Department of Chemistry  Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-012-4623-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(88)90105-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016350510019838"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.07.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-013-2438-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2009.12.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1192\/apt.9.6.456"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602197"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.03.017"},{"key":"e_1_2_7_11_1","first-page":"63","volume":"38","author":"Drug Enforcement Administration","year":"2005","journal-title":"Microgram Bulletin"},{"key":"e_1_2_7_12_1","unstructured":"Drug Enforcement Administration 2013.Available from:http:\/\/www.deadiversion.usdoj.gov\/drug_chem_info\/Khat.pdf(accessed on 02.08.2014)."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.11.012"},{"key":"e_1_2_7_14_1","first-page":"51","article-title":"Experience in the control of Khat\u2010chewing in Somalia","volume":"39","author":"Elmi AS","year":"1987","journal-title":"Bulletin on Narcotics"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-009-1259-7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2007.12.033"},{"key":"e_1_2_7_17_1","unstructured":"FitzgeraldJ.Khat: a literature review 2009. 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