{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:56:13Z","timestamp":1760061373791,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,1,3]],"date-time":"2016-01-03T00:00:00Z","timestamp":1451779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:p>Lychnopholide is a sesquiterpene lactone usually obtained from <jats:italic>Lychnophora<\/jats:italic> and <jats:italic>Eremanthus<\/jats:italic> species and has pharmacological activities that include anti\u2010inflammatory and anti\u2010tumor. Lychnopholide isolated from <jats:italic>Eremanthus matogrossenssis<\/jats:italic> was analyzed in this study. The aims of this study were to develop and validate an analytical methodology by LC\u2010MS\/MS and to quantify lychnopholide in rat plasma. Chromatographic separation was achieved on a C<jats:sub>18<\/jats:sub> column using isocratic elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.4\u2009mL\/min. The detection was performed in multiple\u2010reaction monitoring mode using electrospray ionization in positive mode. The method validation was performed in accordance with regulatory guidelines and the results met the acceptance criteria. The linear range of detection was 10\u2013200\u2009ng\/mL (<jats:italic>r<\/jats:italic>\u2009&gt;\u00a00.9961). The intra\u2010 and inter\u2010day assay variability were &lt;6.2 and &lt;11.7%, respectively. The extraction recovery was approximately 63% using liquid\u2013liquid extraction with chloroform. Lychnopholide was detected in plasma up to 60\u2009min after intravenous administration in rats. This rapid and sensitive method for the analysis of the sesquiterpene lactone lychnopholide in rat plasma can be applied to pharmacokinetic studies of this compound. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3654","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T00:01:16Z","timestamp":1447804876000},"page":"1092-1096","source":"Crossref","is-referenced-by-count":2,"title":["Rapid and efficient method for the quantification of lychnopholide in rat plasma by liquid chromatography\u2013tandem mass spectrometry for pharmacokinetic application"],"prefix":"10.1002","volume":"30","author":[{"given":"Larissa","family":"Lachi\u2010Silva","sequence":"first","affiliation":[{"name":"Preclinical Pharmacokinetic Laboratory, Pharmacy Department Maringa State University  Maring\u00e1\u2013 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Paulo Barreto","family":"Sousa","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Produtos Naturais e Sint\u00e9ticos, Physics and Chemistry Department, College of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo  Ribeir\u00e3o Preto\u2013 SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maiara Camotti","family":"Montanha","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa Cl\u00ednica e Bioequival\u00eancia, University Hospital Maringa State University  Maring\u00e1\u2013 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherwin K. B.","family":"Sy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, College of Pharmacy University of Florida  Gainesville\u2013 Florida USA"},{"name":"Biostatistics Graduate Program Maringa State University  Maring\u00e1\u2013 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Luis Callegari","family":"Lopes","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Produtos Naturais e Sint\u00e9ticos, Physics and Chemistry Department, College of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo  Ribeir\u00e3o Preto\u2013 SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise Brentan","family":"Silva","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Produtos Naturais e Sint\u00e9ticos, Physics and Chemistry Department, College of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo  Ribeir\u00e3o Preto\u2013 SP Brazil"},{"name":"Laborat\u00f3rio de Produtos Naturais e Espectrometria de Massas, Center of Biological and Health Sciences Mato Grosso do Sul Federal University  Campo Grande\u2013 MS Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norberto Peporine","family":"Lopes","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa em Produtos Naturais e Sint\u00e9ticos, Physics and Chemistry Department, College of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo  Ribeir\u00e3o Preto\u2013 SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a","family":"Diniz","sequence":"additional","affiliation":[{"name":"Preclinical Pharmacokinetic Laboratory, Pharmacy Department Maringa State University  Maring\u00e1\u2013 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elza","family":"Kimura","sequence":"additional","affiliation":[{"name":"N\u00facleo de Pesquisa Cl\u00ednica e Bioequival\u00eancia, University Hospital Maringa State University  Maring\u00e1\u2013 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.11.018"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140612780"},{"volume-title":"Sesquiterpene Lactones: Structural Diversity and their Biological Activities. 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