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After liquid\u2013liquid extraction with dichloromethane, separation was achieved on a Syncronis HPLC C<jats:sub>18<\/jats:sub> column (150\u2009mm\u2009\u00d7\u20094.6\u2009mm, 5\u2009\u03bcm) using an isocratic mobile phase system consisting of acetonitrile\u2013water (85:15, v\/v) at a flow rate of 0.6\u2009mL\/min with a split ratio of 1:2. Detection was performed on a TSQ Quantum Ultra mass spectrometer equipped with an positive\u2010ion electrospray ionization source. The lower limits of quantification (LLOQs) were 0.25 and 0.15\u2009ng\/mL for cuIIa and cuIIb, respectively. The intra\u2010 and inter\u2010day precision was &lt;11.5% for the LLOQs and each quality control level of the analytes, and accuracy was between \u22129.1 and 7.6%. The extraction recoveries of the analytes and IS from rat plasma were all &gt;87.1%. The method was fully validated and applied to compare the pharmacokinetic profiles of the two cucurbitacins in rat plasma after oral administration of <jats:italic>H. amabilis<\/jats:italic> extract between normal and indomethacin\u2010induced rats. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3733","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T14:25:31Z","timestamp":1460298331000},"page":"1632-1640","source":"Crossref","is-referenced-by-count":13,"title":["Simultaneous determination of cucurbitacin IIa and cucurbitacin IIb of <i>Hemsleya amabilis<\/i> by HPLC\u2013MS\/MS and their pharmacokinetic study in normal and indomethacin\u2010induced rats"],"prefix":"10.1002","volume":"30","author":[{"given":"Shougang","family":"Wang","sequence":"first","affiliation":[{"name":"Affiliated Hospital of Beihua University  Jilin 132011 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Guan","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Beihua University  Jilin 132011 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuhong","family":"Zhong","sequence":"additional","affiliation":[{"name":"Jilin Medical University  Jilin 132013 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiping","family":"Yang","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Beihua University  Jilin 132011 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weili","family":"Huang","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Beihua University  Jilin 132011 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baoyang","family":"Jia","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Beihua University  Jilin 132011 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Cui","sequence":"additional","affiliation":[{"name":"Affiliated Hospital of Beihua University  Jilin 132011 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.12816\/0006025"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3032"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.10"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2014.11.017"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2014.01.014"},{"key":"e_1_2_6_7_1","first-page":"539","volume-title":"Pharmacopoeia of People's Republic of China (I)","author":"Committee of National Pharmacopoeia, 2005","year":"2005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2013.03.013"},{"key":"e_1_2_6_9_1","first-page":"509","article-title":"Cucurbitacin IIb induces apoptosis of concanavalin A\u2010activated mouse lymphocytes","volume":"30","author":"Hu B","year":"2014","journal-title":"Chinese Journal of Pathophysiology"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2010.09.007"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2006.07.068"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.06.041"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.9866"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(88)90166-7"},{"key":"e_1_2_6_15_1","first-page":"301","article-title":"Experimental research on effects of Xue Dan Wei Chang pill on gastric ulcer","volume":"21","author":"Liu YJ","year":"2010","journal-title":"Lishizhen Medicine & Materia Medica Research"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.02.023"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-012-1921-z"},{"key":"e_1_2_6_18_1","first-page":"76","article-title":"Determination of cucurbitacin IIa in Hemsleya zhejiangensis by high performance liquid chromatography","volume":"10","author":"Shen XT","year":"2012","journal-title":"Guide of China Medicine"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-010-9528-x"},{"key":"e_1_2_6_20_1","first-page":"337","article-title":"Studies on the active antitumor constituents from Hemsleya sinesis","volume":"20","author":"Tao CY","year":"1999","journal-title":"Academic Journal of Second Military Medical University"},{"key":"e_1_2_6_21_1","unstructured":"US Food and Drug Administration.Guidance for Industry:Bioanalytical Method Validation 2013. 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