{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:07:45Z","timestamp":1773799665499,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,5,15]],"date-time":"2016-05-15T00:00:00Z","timestamp":1463270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We applied a new technique for quantitative linear range shift using in\u2010source collision\u2010induced dissociation (CID) to complex biological fluids to demonstrate its utility. The technique was used in a simultaneous quantitative determination method of 5\u2010fluorouracil (5\u2010FU), an anticancer drug for various solid tumors, and its metabolites in human plasma by liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry (LC\/ESI\u2010MS\/MS). To control adverse effects after administration of 5\u2010FU, it is important to monitor the plasma concentration of 5\u2010FU and its metabolites; however, no simultaneous determination method has yet been reported because of vastly different physical and chemical properties of compounds. We developed a new analytical method for simultaneously determining 5\u2010FU and its metabolites in human plasma by LC\/ESI\u2010MS\/MS coupled with the technique for quantitative linear range shift using in\u2010source CID. Hydrophilic interaction liquid chromatography using a stationary phase with zwitterionic functional groups, phosphorylcholine, was suitable for separation of 5\u2010FU from its nucleoside and interfering endogenous materials. The addition of glycerin into acetonitrile\u2010rich eluent after LC separation improved the ESI\u2010MS response of high polar analytes. Based on the validation results, linear range shifts by in\u2010source CID is the reliable technique even with complex biological samples such as plasma. Copyright \u00a9 2016 John Wiley &amp; Sons Ltd.<\/jats:p>","DOI":"10.1002\/bmc.3743","type":"journal-article","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T21:19:00Z","timestamp":1460668740000},"page":"1882-1886","source":"Crossref","is-referenced-by-count":31,"title":["A simultaneous determination method for 5\u2010fluorouracil and its metabolites in human plasma with linear range adjusted by in\u2010source collision\u2010induced dissociation using hydrophilic interaction liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"30","author":[{"given":"Hideaki","family":"Ishii","sequence":"first","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"},{"name":"Laboratory of Racing Chemistry  1731-2 Tsuruta-machi Utsunomiya 320-0851 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Shimada","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"},{"name":"Department of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"},{"name":"Department of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nariyasu","family":"Mano","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"},{"name":"Department of Pharmaceutical Sciences Tohoku University Hospital  1-1 Seiryo-machi, Aoba-ku Sendai 980-8574 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685637"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clpt.2006.06.007"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3633-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6777"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.327"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.11.033"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600671055"},{"key":"e_1_2_6_9_1","unstructured":"EMA.Guideline on bioanalytical method validation 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