{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T02:42:50Z","timestamp":1767926570669,"version":"3.49.0"},"reference-count":6,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T00:00:00Z","timestamp":1466640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A sensitive LC\u2013MS\/MS method was developed and validated for quantitation of saroglitazar using turboion spray interface with positive ion mode. A liquid\u2013liquid extraction, with a mixture of dichloromethane and diethyl ether, was employed for the extraction of saroglitazar and glimepiride (IS) from human plasma. The chromatographic separation was achieved using an ACE\u20105, C<jats:sub>18<\/jats:sub> (4.6\u2009\u00d7\u2009100\u2009mm) column with a gradient mobile phase comprising acetonitrile and ammonium acetate buffer with trifluoracetic acid in purified water. Both analytes were separated within 10\u2009min with retention times of 4.52 and 2.57\u2009min for saroglitazar and IS, respectively. Saroglitazar quantitation was achieved by the summation of two MRM transition pairs (<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 440.2 to <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 366.0 and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 440.2 to <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 183.1), while that of IS was achieved using transition pair <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 491.3 to <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 352.0. The calibration standards of saroglitazar showed linearity from 0.2 to 500\u2009ng\/mL, with a lower limit of quantitation of 0.2\u2009ng\/mL. The biases for inter\u2010 and intra\u2010batch assays were \u22127.51\u20131.15% and \u221211.21 to \u22123.25%, respectively, while the corresponding precisions were 5.04\u20138.06% and 1.53\u20137.68%, respectively. The developed method was used to monitor the plasma concentrations of saroglitazar in clinical samples.<\/jats:p>","DOI":"10.1002\/bmc.3760","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T11:08:45Z","timestamp":1463483325000},"page":"1900-1907","source":"Crossref","is-referenced-by-count":2,"title":["Quantitative determination of saroglitazar, a predominantly PPAR alpha agonist, in human plasma by a LC\u2013MS\/MS method utilizing electrospray ionization in a positive mode"],"prefix":"10.1002","volume":"30","author":[{"given":"Ashok","family":"Ghoghari","sequence":"first","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjeet","family":"Dash","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandrakant","family":"Bhatt","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanchan","family":"Singh","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anil","family":"Jha","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harilal","family":"Patel","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul","family":"Gupta","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Clinical Research  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevinkumar","family":"Kansagra","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Clinical Research  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuggehally R.","family":"Srinivas","sequence":"additional","affiliation":[{"name":"Zydus Research Centre Bioanalytical Laboratory  Ahmedabad \u2010382 210 Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146222"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/prp2.136"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40261-013-0128-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/dia.2013.0253"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1932296813518680"},{"key":"e_1_2_7_7_1","unstructured":"US DHHS FDA and CDER.Guidance for Industry:Bioanalytical Method Validation. US Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research and Center for Veterinary Medicine May2001."}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3760","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3760","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:05:05Z","timestamp":1760036705000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,23]]},"references-count":6,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1002\/bmc.3760"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3760","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,23]]}}}