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Serum and gastric juice were prepared with a simple protein precipitation using aqueous 0.1% formic acid and acetonitrile. As a result, it was found that the kynurenine pathway of tryptophan metabolism was activated in gastric cancer and that the metabolic ratio of kynurenine\/tryptophan, which reflects the enzyme activity of indoleamine\u20102,3\u2010dioxygenase, was associated with the observed metabolic changes. Finally, the investigation of tryptophan metabolites, especially kynurenic acid, in serum and gastric juice might serve as biomarkers for gastric cancer. The findings in this study provide critical information of tryptophan metabolism which can be applied to a serum\u2010based diagnostic test for gastric cancer.<\/jats:p>","DOI":"10.1002\/bmc.3773","type":"journal-article","created":{"date-parts":[[2016,5,30]],"date-time":"2016-05-30T11:42:49Z","timestamp":1464608569000},"page":"1963-1974","source":"Crossref","is-referenced-by-count":56,"title":["Development of simultaneous analysis of tryptophan metabolites in serum and gastric juice \u2013 an investigation towards establishing a biomarker test for gastric cancer diagnosis"],"prefix":"10.1002","volume":"30","author":[{"given":"Jong Min","family":"Choi","sequence":"first","affiliation":[{"name":"Molecular Recognition Research Center Korea Institute of Science and Technology  Seoul Republic of Korea"},{"name":"Graduate School of Pharmaceutical Sciences Ewha Womans University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won 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