{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:52:38Z","timestamp":1760061158907,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,18]],"date-time":"2016-09-18T00:00:00Z","timestamp":1474156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501367","31270335"],"award-info":[{"award-number":["31501367","31270335"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dihydroartemisinic acid (DHAA) is the direct precursor to artemisinin, an effective anti\u2010malaria compound from <jats:italic>Artemisia annua<\/jats:italic> L. (<jats:italic>A. annua<\/jats:italic>), and it can be transformed to artemisinin without the catalysis of enzyme. A rapid and sensitive analysis of DHAA in <jats:italic>A. annua<\/jats:italic> is needed to screen excellent plant resources aimed to improve artemisinin production. In order to develop a rapid and sensitive determination method for DHAA in plant, the extraction and analysis conditions were extensively investigated in the present work. As a result, extraction of powdered <jats:italic>A. annua<\/jats:italic> leaves at 55\u00b0C for 50\u2009min with chloroform resulted in the highest yield of DHAA, with a recovery of &gt;98%. The precision of this gas chromatographic procedure ranged from 1.22 to 2.94% for intra\u2010day and from 1.69 to 4.31% for inter\u2010day, respectively. The accuracy was 99.55\u2013103.02% for intra\u2010day and 98.86\u201399.98% for inter\u2010day, respectively. The measured LOQ and LOD values of the proposed method reached 5.00 and 2.00\u2009\u03bcg\/mL, respectively. Validation indicated the method was robust, quick, sensitive and adequate for DHAA analysis.<\/jats:p>","DOI":"10.1002\/bmc.3824","type":"journal-article","created":{"date-parts":[[2016,8,24]],"date-time":"2016-08-24T10:12:37Z","timestamp":1472033557000},"source":"Crossref","is-referenced-by-count":3,"title":["Determination of dihydroartemisinic acid in <i>Artemisia annua<\/i> L. by gas chromatography with flame ionization detection"],"prefix":"10.1002","volume":"31","author":[{"given":"Na","family":"Tian","sequence":"first","affiliation":[{"name":"Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Tea Science, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongming","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Tea Science, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"},{"name":"Department of Tea Science, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuoqian","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"},{"name":"Department of Tea Science, College of Horticulture and Hardening Hunan Agricultural University  Changsha 410128 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15117603"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2003.11.070"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1075155"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1101"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf903222j"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1079\/PGR200585"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1182612"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082962"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4324\/9780203303061_chapter_10"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.02.089"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.09.023"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.953"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1184780"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2006.08.043"},{"issue":"8","key":"e_1_2_7_16_1","first-page":"701","article-title":"Genetic 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