{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T20:36:18Z","timestamp":1778358978189,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,29]],"date-time":"2016-09-29T00:00:00Z","timestamp":1475107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["9313"],"award-info":[{"award-number":["9313"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Adhatoda beddomei<\/jats:italic> and <jats:styled-content style=\"fixed-case\"><jats:italic>Adhatoda vasica<\/jats:italic><\/jats:styled-content> leaf, known as \u2018<jats:italic>Vasaka<\/jats:italic>\u2019 and\/or \u2018<jats:italic>Vasa<\/jats:italic>\u2019 in Ayurveda and \u2018Malabar nut\u2019 in English, is an official drug in the <jats:italic>Indian Pharmacopoeia<\/jats:italic>. The medicinal properties of these plants are due to the presence of pyrroquinazoline alkaloids. An UHPLC\u2013ESI\/MS\/MS method in both positive and negative electrospray ionization in multiple\u2010reaction\u2010monitoring mode was developed and validated for the estimation of alkaloids and flavonoids in <jats:italic>Adhatoda<\/jats:italic> species and their marketed herbal formulations. Chromatographic separation was achieved on an Acquity UPLC\u00ae BEH C<jats:sub>18<\/jats:sub>\u2010column using a gradient elution with 0.1% formic acid in water and methanol. The developed method was validated as per International Conference on Harmonization guidelines and found to be accurate with overall recovery in the range 94.2\u2013105.0% (RSD\u2009\u2264\u20091.71%), precise (RSD\u2009\u2264\u20093.44%) and linear (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009\u2265\u20090.9992) over the concentration range of 0.5\u20131000\u2009ng\/mL. The total content of alkaloids and flavonoids were highest in the chloroform and aqueous fraction of <jats:styled-content style=\"fixed-case\"><jats:italic>A. vasica<\/jats:italic><\/jats:styled-content> leaf, respectively. The results indicated that the developed method was simple, rapid, sensitive, selective and accurate for the estimation of multiple bioactive constituents in crude mixture, and therefore could make a contribution to the quality control of <jats:italic>Adhatoda<\/jats:italic> species and its derived herbal formulations.<\/jats:p>","DOI":"10.1002\/bmc.3827","type":"journal-article","created":{"date-parts":[[2016,8,26]],"date-time":"2016-08-26T21:22:43Z","timestamp":1472246563000},"source":"Crossref","is-referenced-by-count":7,"title":["Simultaneous determination of pyrroquinazoline alkaloids and flavonoids in <i>Adhatoda beddomei<\/i> and <scp><i>Adhatoda vasica<\/i><\/scp> and their marketed herbal formulations using ultra\u2010high\u2010performance liquid chromatography coupled with triple quadrupole linear ion trap mass spectrometry"],"prefix":"10.1002","volume":"31","author":[{"given":"Awantika","family":"Singh","sequence":"first","affiliation":[{"name":"Sophisticated Analytical Instrument Facility Division CSIR\u2010Central Drug Research Institute  Lucknow India"},{"name":"Academy of Scientific and Innovative Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility Division CSIR\u2010Central Drug Research Institute  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vikas","family":"Bajpai","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility Division CSIR\u2010Central Drug Research Institute  Lucknow India"},{"name":"Academy of Scientific and Innovative Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brijesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Sophisticated Analytical Instrument Facility Division CSIR\u2010Central Drug Research Institute  Lucknow India"},{"name":"Academy of Scientific and Innovative Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)96410-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.737"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70005-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)94396-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(99)00074-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02234869"},{"key":"e_1_2_8_8_1","first-page":"235","article-title":"Development and validation of reverse phase high performance liquid chromatography method for quantitative estimation of vasicine in bulk and pharmaceutical dosage form","volume":"7","author":"Farooqui N. 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A.","year":"2013","journal-title":"Indo American Journal of Pharmaceutical Research"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.127375"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.11.049"},{"key":"e_1_2_8_16_1","first-page":"3","article-title":"Medicinal plants of wide use in India with special reference to Sitapur district (Uttar Pradesh)","volume":"65","author":"Siddiqui M. B.","year":"1994","journal-title":"Fitoterapia"},{"key":"e_1_2_8_17_1","first-page":"322","article-title":"Ethnobotanical uses of medicinal plant, \n                     Justicia adhatoda\n                   L. by Meitei community of Manipur, India","volume":"1","author":"Singh K. 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Extracts against UTI causing microorganism","volume":"27","author":"Singh M.","year":"2014","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201500063"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7202"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7126"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/0250-474X.40329"},{"key":"e_1_2_8_24_1","first-page":"280","article-title":"Influence of drying temperature on quality parameters of \n                     Adhatoda vasica\n                   leaves","volume":"2","author":"Srinivasan K.","year":"2014","journal-title":"International Journal of Life Sciences Biotechnology and Pharma Research"},{"key":"e_1_2_8_25_1","first-page":"55","article-title":"GC\u2013MS profiling and in vitro radical scavenging effect of Adhatoda beddomei","volume":"2","author":"Srinivasan M.","year":"2014","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2005.09.010"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3827","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:01:14Z","timestamp":1760043674000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,29]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/bmc.3827"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3827","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,29]]},"article-number":"e3827"}}