{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:25:20Z","timestamp":1761513920485,"version":"build-2065373602"},"reference-count":10,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T00:00:00Z","timestamp":1485302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2017,7]]},"abstract":"Abstract<\/jats:title>Acotiamide hydrochloride (ACT) is a drug used for the treatment of functional dyspepsia. Understanding which metabolites are likely to be formed in vivo<\/jats:italic> is essential for interpreting pharmacology, pharmacokinetic and toxicology data. The metabolism of ACT has been investigated using a specific and sensitive liquid chromatography positive ion electrospray ionization high\u2010resolution tandem mass spectrometry method. In vivo<\/jats:italic> samples including rat plasma, urine and feces were collected separately after dosing healthy Sprague\u2013Dawley rats at a dose of 20\u2009mg\u2009kg \u22121<\/jats:sup> ACT at different time points up to 24\u2009h. The metabolites were enriched by optimized sample preparation involving protein precipitation using acetonitrile followed by solid\u2010phase extraction. The mass defect filter technique was used for better detection of both predicted and unexpected drug metabolites with the majority of interference ions removed. The structural elucidation of the metabolites was performed by comparing their [M\u2009+\u2009H]+<\/jats:sup> ions and their product ions with those of the parent drug. As a result, a total of seven hitherto unknown metabolites were characterized from the biosamples. The only phase I metabolite detected was N\u2010<\/jats:italic>despropyl acotiamide, whereas six phase II glucuronide conjugate metabolites were identified.<\/jats:p>","DOI":"10.1002\/bmc.3915","type":"journal-article","created":{"date-parts":[[2016,12,10]],"date-time":"2016-12-10T14:12:45Z","timestamp":1481379165000},"source":"Crossref","is-referenced-by-count":3,"title":["In vivo<\/i> metabolite identification of acotiamide in rats using ultra\u2010performance liquid chromatography\u2013quadrupole\/time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"31","author":[{"given":"Prinesh N.","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradipbhai D.","family":"Kalariya","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohit","family":"Thummar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Gananadhamu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Srinivas","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research Hyderabad Telangana India"},{"name":"National Centre for Mass Spectrometry CSIR\u2010Indian Institute of Chemical Technology Hyderabad Telangana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.06.040"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920011795202910"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.12.022"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301454"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-013-0100-9"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3555"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2010.10.014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.22649"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1610"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2014.08.006"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.3915","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.3915","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:54:38Z","timestamp":1760036078000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.3915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,25]]},"references-count":10,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1002\/bmc.3915"],"URL":"https:\/\/doi.org\/10.1002\/bmc.3915","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"type":"print","value":"0269-3879"},{"type":"electronic","value":"1099-0801"}],"subject":[],"published":{"date-parts":[[2017,1,25]]},"article-number":"e3915"}}