{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T21:20:54Z","timestamp":1780348854799,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,2,5]],"date-time":"2017-02-05T00:00:00Z","timestamp":1486252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study describes a specific, precise, sensitive and accurate method for simultaneous determination of hydroxyzine, loratadine, terfenadine, rupatadine and their main active metabolites cetirizine, desloratadine and fexofenadine, in serum and urine using meclizine as an internal standard. Solid\u2010phase extraction method for sample clean\u2010up and preconcentration of analytes was carried out using Phenomenex Strata\u2010X\u2010C and Strata X polymeric cartridges. Chromatographic analysis was performed on a Phenomenex cyano (150\u2009\u00d7\u20094.6\u2009mm i.d., 5\u2009\u03bcm) analytical column. A D\u2010optimal mixture design methodology was used to evaluate the effect of changes in mobile phase compositions on dependent variables and optimization of the response of interest. The mixture design experiments were performed and results were analyzed. The region of ideal mobile phase composition consisting of acetonitrile\u2013methanol\u2013ammonium acetate buffer (40\u2009m<jats:sc>m<\/jats:sc>; pH\u20093.8 adjusted with acetic acid): 18:36:46% <jats:italic>v<\/jats:italic>\/v\/v was identified by a graphical optimization technique using an overlay plot. While using this optimized condition all analytes were baseline resolved in &lt;10\u2009min. Solvent mixtures were delivered at 1.5\u2009mL\/min flow rate and analytes peaks were detected at 222\u2009nm. The proposed bioanalytical method was validated according to US Food and Drug Administration guidelines. The proposed method was sensitive with detection limits of 0.06\u20130.15\u2009\u03bcg\/mL in serum and urine samples. Relative standard deviation for inter\u2010 and intra\u2010day precision data was found to be &lt;7%. The proposed method may find application in the determination of selected antihistaminic drugs in biological fluids.<\/jats:p>","DOI":"10.1002\/bmc.3932","type":"journal-article","created":{"date-parts":[[2017,1,6]],"date-time":"2017-01-06T00:32:56Z","timestamp":1483662776000},"source":"Crossref","is-referenced-by-count":5,"title":["D\u2010Optimal mixture design optimization of an HPLC method for simultaneous determination of commonly used antihistaminic parent molecules and their active metabolites in human serum and urine"],"prefix":"10.1002","volume":"31","author":[{"given":"Selvakumar","family":"Kanthiah","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Engineering and Technology Annamalai University  Annamalai Nagar Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0276-439X","authenticated-orcid":false,"given":"Valliappan","family":"Kannappan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Engineering and Technology Annamalai University  Annamalai Nagar Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.10.3.547"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1674-8301(11)60048-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-009-9285-6"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/0470042206"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2015.06.034"},{"key":"e_1_2_8_7_1","first-page":"301","article-title":"Some pharmacokinetic aspects of the lipophilic terfenadine and zwitterionic fexofenadine in humans","volume":"8","author":"Chen C.","year":"2007","journal-title":"Drugs Reseach and Discovery"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986708785747625"},{"key":"e_1_2_8_9_1","first-page":"600","article-title":"A validated HPLC\u2010ESI\u2010MS method for the determination of loratadine in human plasma and its application to pharmacokinetic studies","volume":"59","author":"Chen J.","year":"2004","journal-title":"Pharmazie"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AY00384E"},{"key":"e_1_2_8_11_1","first-page":"3","article-title":"Comparative pharmacology of the H1 antihistamines","volume":"16","author":"Cuvillo A. 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