{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:57:49Z","timestamp":1760889469994,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T00:00:00Z","timestamp":1507593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tanreqing Injection (TRQ) has been used primarily in treating infections of the upper respiratory tract and serious influenza in China, as a classical compound herbal recipe. TRQ had been demonstrated to have effects of clearing heat, eliminating phlegm, detoxification, reducing inflammation and alleviating cough. The survival rate, histopathology of lungs and viral titers in mice were evaluated in this study to verify the curative effect of TRQ. However, there is not enough information about the components. In the present study, a high\u2010performance and practical LC\/QTOF\/MS method was developed for characterization and identification of the natural ingredients in TRQ. A total of 60 compounds, including 10 amino acids, 10 iridoid glucosides, 14 flavonoids, 13 other phenolic compounds, 10 steroid acids and three other compounds, were characterized and identified. We also confirmed the material basis of anti\u2010Influenza A active ingredients in TRQ. Therefore, we have developed an accurate analytical method. LC\/QTOF\/MS could be applied for identification the complex components in traditional Chinese medicine.<\/jats:p>","DOI":"10.1002\/bmc.4097","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T09:37:59Z","timestamp":1505727479000},"source":"Crossref","is-referenced-by-count":15,"title":["Material basis studies of anti\u2010Influenza A active ingredients in Tanreqing Injection"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8109-6678","authenticated-orcid":false,"given":"Haiyan","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Microbiological and Biochemical Pharmacy, School of Pharmacy Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingcang","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Institutes of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xunlong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Microbiological and Biochemical Pharmacy, School of Pharmacy Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenchen","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Microbiological and Biochemical Pharmacy, School of Pharmacy Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenggang","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Institutes of Materia Medica, Chinese Academy of Sciences  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,10]]},"reference":[{"issue":"18","key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1056\/NEJMra1000449","article-title":"Clinical aspects of pandemic 2009 influenza A (H1N1) virus infection","volume":"362","author":"Bautista E.","year":"2010","journal-title":"New England Journal of Medicine"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(96)00636-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2012.09.038"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2015.11.013"},{"issue":"6","key":"e_1_2_6_6_1","first-page":"521","article-title":"Simultaneous quantification of ten phenolic acids in Lonicerae Japonicae Flos by HPLC\u2010DAD","volume":"22","author":"Chen K.","year":"2013","journal-title":"Journal of Chinese Pharmaceutical Sciences"},{"key":"e_1_2_6_7_1","article-title":"Role of baicalin in anti\u2010influenza virus A as a potent inducer of IFN\u2010gamma","author":"Chu M.","year":"2015","journal-title":"Biomed Research International, 2015"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0903810"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2192-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-8670-x"},{"issue":"11","key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.3987\/R-1982-11-2033","article-title":"Structures of forsythoside C and D, antibacterial principles of Forsythia suspensa fruits","volume":"19","author":"Endo K.","year":"1982","journal-title":"Heterocycles"},{"issue":"10","key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1139\/v84-343","article-title":"Structures of rengyol, rengyoxide and rengyolone, new cyclohexylethane, derivatives from Forsythia suspensa fruits","volume":"62","author":"Endo K.","year":"1984","journal-title":"Canadian Journal of Chemistry"},{"issue":"4","key":"e_1_2_6_13_1","first-page":"581","article-title":"Phenolic compounds from Scutellaria pontica","volume":"26","author":"Ersoz T.","year":"2002","journal-title":"Turkish Journal of Chemistry"},{"issue":"1","key":"e_1_2_6_14_1","first-page":"1","article-title":"Antiviral agents for the treatment and chemoprophylaxis of influenza \u2013 Recommendations of the Advisory Committee on Immunization Practices (ACIP)","volume":"60","author":"Fiore A. 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