{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:43:33Z","timestamp":1790343813740,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T00:00:00Z","timestamp":1514246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81503013"],"award-info":[{"award-number":["81503013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reliable methods for the determination of tryptophan and its metabolites are vital to the monitoring of biochemical states during the initiation and progression of cardiovascular disease. In the present study, a single\u2010run liquid chromatography\u2013tandem mass spectrometry (LC\u2010MS\/MS) method was developed for the simultaneous determination of tryptophan (Trp) and its metabolites, including kynurenine (Kyn), kynurenic acid (KA), xanthurenic acid (XA) and 5\u2010hydroxytryptamine (5\u2010HT), in human plasma. The plasma samples were prepared using a protein precipitation approach, and the chromatographic separation was performed by gradient elution on a C<jats:sub>18<\/jats:sub> column within a total analysis time of 3.5\u2009min. The calibration ranges were 40\u201320,000\u2009ng\/mL for Trp, 4\u20132000\u2009ng\/mL for Kyn, 0.2\u2013100\u2009ng\/mL for KA, 0.4\u2013200\u2009ng\/mL for XA and 1\u2013500\u2009ng\/mL for 5\u2010HT, and the precision and accuracy were acceptable. The evaluation of recovery and internal standard\u2010normalized matrix effect proved that the sample preparation approach was effective and the matrix effect could be negligible. The newly developed method was successfully applied to the analysis of plasma samples from healthy individuals and myocardial infarction patients. The findings suggested that the plasma concentrations of Trp, Kyn, 5\u2010HT as well as the concentration ratios of Kyn\/Trp and Trp\/5\u2010HT might serve as biomarkers for the monitoring of acute myocardial infarction.<\/jats:p>","DOI":"10.1002\/bmc.4156","type":"journal-article","created":{"date-parts":[[2017,11,29]],"date-time":"2017-11-29T10:23:29Z","timestamp":1511951009000},"source":"Crossref","is-referenced-by-count":63,"title":["Simultaneous determination of tryptophan, kynurenine, kynurenic acid, xanthurenic acid and 5\u2010hydroxytryptamine in human plasma by LC\u2010MS\/MS and its application to acute myocardial infarction monitoring"],"prefix":"10.1002","volume":"32","author":[{"given":"Qing","family":"Tong","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Song","sequence":"additional","affiliation":[{"name":"The Center for Biomedical Research, Tongji Hospital Affiliated with Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjie","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6445-6750","authenticated-orcid":false,"given":"Jianguo","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Tongji Hospital Affiliated with Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acvd.2016.08.002"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00485"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3773"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2506"},{"key":"e_1_2_8_6_1","unstructured":"European Medicines Agency(2011).Guideline on bioanalytical method validation.http:\/\/www.ema.europa.eu\/docs\/en_GB\/document_library\/Scientific_guideline\/2011\/08\/WC500109686.pdf."},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2015.03.413"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio-2016-0111"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2423(07)00007-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkw071"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-015-1077-9"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2005.12.004"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0752-7"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2016.01.031"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867321666140304105526"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.01.023"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2008.11.003"},{"key":"e_1_2_8_18_1","first-page":"698","article-title":"Systemic markers of interferon\u2010\u03b3\u2010mediated immune activation and long\u2010term prognosis in patients with stable coronary artery disease. Arteriosclerosis Thrombosis and Vascular","volume":"31","author":"Pedersen E. R.","year":"2011","journal-title":"Biology"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.304674"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.5482\/HAMO-14-10-0052"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/157016106777698379"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2005.06.013"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12430"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2012.12.090"},{"key":"e_1_2_8_25_1","unstructured":"US Food and Drug Administration(2013).Guidance for Industry: Bioanalytical Method Validation.http:\/\/www.fda.gov\/downloads\/drugs\/guidancecomplianceregulatoryinformation\/guidances\/ucm368107.pdf."},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2092"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"314","DOI":"10.3945\/jn.116.241711","article-title":"Increases in plasma tryptophan are inversely associated with incident cardiovascular disease in the prevenci\u00f3n con dieta mediterr\u00e1nea (predimed) study","volume":"147","author":"Yu E.","year":"2017","journal-title":"Journal of Nutrition"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4156","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4156","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:42:36Z","timestamp":1760038956000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4156"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,26]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1002\/bmc.4156"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4156","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,26]]},"article-number":"e4156"}}