{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T16:41:16Z","timestamp":1779208876931,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T00:00:00Z","timestamp":1518393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81303300"],"award-info":[{"award-number":["81303300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Stellera chamaejasme<\/jats:italic> L. has been used as a traditional Chinese medicine for the treatment of scabies, tinea, stubborn skin ulcers, chronic tracheitis, cancer and tuberculosis. A sensitive and selective ultra\u2010high liquid chromatography\u2013tandem mass spectrometry (UPLC\u2010MS\/MS) method was developed and validated for the simultaneous determination of five flavonoids (stelleranol, chamaechromone, neochamaejasmin A, chamaejasmine and isochamaejasmin) of <jats:italic>S. chamaejasme<\/jats:italic> L. in rat plasma. Chromatographic separation was accomplished on an Agilent Poroshell 120 EC\u2010C<jats:sub>18<\/jats:sub> column (2.1 \u00d7 100\u2009mm, 2.7\u2009\u03bcm) with gradient elution at a flow rate of 0.4\u2009mL\/min and the total analysis time was 7\u2009min. The analytes were detected using multiple reaction monitoring in positive ionization mode. The samples were prepared by liquid\u2013liquid extraction with ethyl acetate. The UPLC\u2010MS\/MS method was validated for specificity, linearity, sensitivity, accuracy and precision, recovery, matrix effect and stability. The validated method exhibited good linearity (<jats:italic>r \u2265<\/jats:italic> 0.9956), and the lower limits of quantification ranged from 0.51 to 0.64\u2009ng\/mL for five flavonoids. The intra\u2010 and inter\u2010day precision were both &lt;10.2%, and the accuracy ranged from \u221211.79 to 9.21%. This method was successfully applied to a pharmacokinetic study of five flavonoids in rats after oral administration of ethyl acetate extract of <jats:italic>S. chamaejasme<\/jats:italic> L.<\/jats:p>","DOI":"10.1002\/bmc.4189","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T10:32:43Z","timestamp":1515753163000},"source":"Crossref","is-referenced-by-count":8,"title":["A UPLC\u2010MS\/MS method for simultaneous determination of five flavonoids from <i>Stellera chamaejasme<\/i> L. in rat plasma and its application to a pharmacokinetic study"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3463-6560","authenticated-orcid":false,"given":"Yun\u2010Qing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"},{"name":"Department of Pharmacy The Children Hospital of Shanxi  Taiyuan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-4778","authenticated-orcid":false,"given":"Cheng\u2010Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing\u2010qing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Science, College of Pharmacy University of Michigan  Ann Arbor Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3129-872X","authenticated-orcid":false,"given":"Liang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"},{"name":"Center of Health Management Zhejiang Changtai Hospital  Zhuji China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/np300815t"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8546-5-23"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.3390\/molecules19021603","article-title":"A new dicoumarinyl ether from the roots of Stellera chamaejasme L","volume":"19","author":"Li J.","year":"2014","journal-title":"Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2014.01.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2014.05.042"},{"key":"e_1_2_7_7_1","first-page":"404","article-title":"In vitro inhibitory and pro\u2010apoptotic effect of Stellera chamaejasme L extract on human lung cancer cell line NCI\u2010H157","volume":"32","author":"Liu X.","year":"2012","journal-title":"Journal of Traditional Chinese Medicine\/Chung i tsa chih ying wen pan"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-116"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2012.643"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.04.009"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"2931","DOI":"10.3390\/molecules20022931","article-title":"Inhibitory effects of neochamaejasmin B on P\u2010glycoprotein in MDCK\u2010hMDR1 cells and molecular docking of NCB binding in P\u2010glycoprotein","volume":"20","author":"Pan L.","year":"2015","journal-title":"Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1177\/1934578X1501000115","article-title":"Diversity in the flavonoid composition of Stellera chamaejasme in the Hengduan Mountains","volume":"10","author":"Shirai K.","year":"2015","journal-title":"Natural Product Communications"},{"key":"e_1_2_7_13_1","unstructured":"US Food and Drug Administration(2013).Guidance for Industry: Bioanalytical Method Validation. Retrieved fromhttp:\/\/www.fda.gov\/downloads\/drugs\/guidancecomplianceregulatoryinformation\/guidances\/ucm070107.pdf"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-015-0082-6"},{"key":"e_1_2_7_15_1","first-page":"669","article-title":"New biflavanones and bioactive compounds from Stellera chamaejasme L","volume":"36","author":"Xu Z. H.","year":"2001","journal-title":"Yao xue xue bao\/Acta Pharmaceutica Sinica"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2014.07.013"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2014.1001225"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3209-5"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200890130"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"8165","DOI":"10.3390\/molecules16108165","article-title":"Chamaejasmine induces apoptosis in human lung adenocarcinoma A549 cells through a Ros\u2010mediated mitochondrial pathway","volume":"16","author":"Yu H.","year":"2011","journal-title":"Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2012.158"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(15)30063-7"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"845","DOI":"10.3390\/molecules18010845","article-title":"Chamaejasmine arrests cell cycle, induces apoptosis and inhibits nuclear NF\u2010\u03baB translocation in the human breast cancer cell line MDA\u2010MB\u2010231","volume":"18","author":"Zhang T.","year":"2013","journal-title":"Molecules: A Journal of Synthetic Chemistry and Natural Product Chemistry"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2564"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4189","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4189","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:30:35Z","timestamp":1760041835000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,12]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/bmc.4189"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4189","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,12]]},"article-number":"e4189"}}