{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T21:24:32Z","timestamp":1771881872794,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T00:00:00Z","timestamp":1519084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Qing Lan Project of Jiangsu Province","award":["2017"],"award-info":[{"award-number":["2017"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No.81403314"],"award-info":[{"award-number":["No.81403314"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Open Project Program of Guangxi Key Laboratory of Tradtitonal Chinese Medicaine Quality Stardands","award":["No.201503"],"award-info":[{"award-number":["No.201503"]}]},{"name":"the Natural Research Foundation of Jiangsu Province","award":["No. BK20161456"],"award-info":[{"award-number":["No. BK20161456"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rhizoma et Radix Polygoni Cuspidati (RRPC) is commonly prescribed for the treatment of amenorrhea, arthralgia, jaundice and abscess in traditional Chinese medicine. Previous pharmacological studies have indicated that polyphenols are the main pharmacological active ingredients in RRPC. Meanwhile, the poor bioavailability of polyphenols in RRPC implies that those components are probably metabolized by intestinal bacteria before absorption. However, there is rather limited information about RRPC''s metabolites produced by intestinal bacteria and the intestinal absorbed constituents. In the present study, the metabolites were characterized after the aqueous extract of RRPC was incubated with the crude enzyme of human intestinal bacteria <jats:italic>in vitro<\/jats:italic>. The metabolic characteristics of glycosides in RRPC were figured out by comparing the metabolic profiles of emodin\u20108<jats:italic>\u2010O\u2010\u03b2<\/jats:italic>\u2010<jats:sc>d<\/jats:sc>\u2010glucopyranoside and polydatin between aqueous extract of RRPC and equivalent amounts of these two glycosides. The transitional constituents absorbed into blood were investigated in rats via intraduodental administration and portal vein intubation. A total of 38 prototype components and 43 metabolites were detected and characterized <jats:italic>in vivo<\/jats:italic>. The overall results demonstrated that the intestinal bacteria played an important role in the metabolism of RRPC, and the main metabolic pathways were hydrolysis <jats:italic>in vitro<\/jats:italic>, glucuronidation and sulfation <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/bmc.4190","type":"journal-article","created":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T18:12:36Z","timestamp":1516039956000},"source":"Crossref","is-referenced-by-count":24,"title":["Intestinal metabolism of <i>Polygonum cuspidatum in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8823-399X","authenticated-orcid":false,"given":"Jinfeng","family":"Fu","sequence":"first","affiliation":[{"name":"Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University  Nanjing China"},{"name":"Changzhou Center for Food and Drug Control  Changzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9446-9237","authenticated-orcid":false,"given":"Zunjian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University  Nanjing China"},{"name":"State Key Laboratory of Natural Medicine China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0398-8638","authenticated-orcid":false,"given":"Rui","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education) China Pharmaceutical University  Nanjing China"},{"name":"State Key Laboratory of Natural Medicine China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,20]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"G191","article-title":"A simple method for chronic cannulation of the portal vein in intact unrestrained rats","volume":"255","author":"Braillon A.","year":"1988","journal-title":"The American Physiological Society"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2012.07.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2013.08.006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.160455"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.11.015"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032673"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2009.0724"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"14582","DOI":"10.1016\/S0021-9258(18)66910-8","article-title":"Reactivity of a sulfhydryl group of the ras oncogene product p21 modulated by GTP Binding","volume":"31","author":"Hattori S.","year":"1986","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm100274c"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.10.153"},{"key":"e_1_2_8_12_1","first-page":"103","article-title":"Effect of polydatin on free calcium dynamic changes in lipopolysaccharide\u2010stimulated single macrophages","volume":"17","author":"Jin L. 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