{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:53:14Z","timestamp":1761511994493,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,7]],"date-time":"2004-10-07T00:00:00Z","timestamp":1097107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this investigation, the measurement and identi\ufb01cation of the <jats:italic>S<\/jats:italic>\u2010oxidation products of three simple sulphides\u2014ethyl methyl sulphide (EMS), 4\u2010chlorophenyl methyl sulphide (CPMS) and diphenyl sulphide (DPS)\u2014in rat urine were carried out and a study of the effects of phenobarbitone (PB), <jats:italic>\u03b2<\/jats:italic>\u2010naphtho\ufb02avone (<jats:italic>\u03b2<\/jats:italic>NF) and methimazole on the urinary levels of their metabolites was conducted. Male Wistar rats (<jats:italic>n<\/jats:italic> = 4) were pretreated with PB (80 mg\/kg\/day in saline, i.p.), <jats:italic>\u03b2<\/jats:italic>NF (100 mg\/kg\/day in corn oil, i.p.), methimazole (50 mg\/kg\/day in saline, i.p.) or the vehicles alone (1 mL\/kg) for three consecutive days. After pretreatment, EMS, CPMS or DPS (50 mg\/kg in corn oil, 500 \u00b5L) was administered orally to the appropriate groups of rats. The animals were placed in metabolic cages and urine samples collected at 24 h intervals over 96 h. Chromatographic and spectroscopic techniques were used for the measurement and identi\ufb01cation of the sulphoxides and sulphones of EMS, CPMS and DPS in rat urine. Although only a trace of ethyl methyl sulphoxide (EMSO) was present in rat urine after administration of EMS, ethyl methyl sulphone (EMSO<jats:sub>2<\/jats:sub>) accounted for about 16% of the administered dose in the urine of male rats given EMS. In addition, pretreatment of rats with methimazole signi\ufb01cantly decreased the <jats:italic>S<\/jats:italic>\u2010oxidation of EMS. 4\u2010Chlorophenyl methyl sulphone (CPMSO<jats:sub>2<\/jats:sub>) was the main metabolite recovered in the urine of male rats treated with CPMS, accounting for about 10% of the dose. Pretreatment of rats with PB before administration of CPMS signi\ufb01cantly increased the levels of CPMSO<jats:sub>2<\/jats:sub> excreted in the urine. Additionally, pretreatment of rats with methimazole signi\ufb01cantly decreased the <jats:italic>S<\/jats:italic>\u2010oxidation of CPMS <jats:italic>in vivo<\/jats:italic>. About 2.5% of diphenyl sulphoxide (DPSO) and 4% of diphenyl sulphone (DPSO<jats:sub>2<\/jats:sub>) were recovered in the urine of male rats given DPS. Pretreatment of rats with PB, <jats:italic>\u03b2<\/jats:italic>NF or methimazole before administration of DPS decreased the levels of DPSO and DPSO<jats:sub>2<\/jats:sub> excreted in the urine, although this was not statistically signi\ufb01cant. These results indicate that microsomal monooxygenases mediate the <jats:italic>S<\/jats:italic>\u2010oxidation of EMS, CPMS and DPS to their corresponding sulphones via a transient sulphoxide in rats. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.421","type":"journal-article","created":{"date-parts":[[2004,10,7]],"date-time":"2004-10-07T08:01:26Z","timestamp":1097136086000},"page":"87-98","source":"Crossref","is-referenced-by-count":4,"title":["Determination of the sulphoxides and sulphones of three simple sulphides in rat urine: effects of phenobarbitone, <i>\u03b2<\/i>\u2010naphtho\ufb02avone and methimazole"],"prefix":"10.1002","volume":"19","author":[{"given":"Ivo P.","family":"Nnane","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyaquat A.","family":"Damani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,7]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"135","volume-title":"Sulphur\u2010containing Drugs and Related Organic Compounds","author":"Damani LA","year":"1989"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/DMDI.1996.13.1.1"},{"key":"e_1_2_1_4_1","first-page":"629","article-title":"Cytochrome P\u2010450 and non\u2010cytochrome P\u2010450 sulphoxidations","volume":"36","author":"Hoodi AA","year":"1984","journal-title":"Journal of Pharmaceutics and Pharmacology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/209619a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S0090-9556(25)07818-3","article-title":"Loss of rat liver microsomal cytochrome P\u2010450 during methimazole metabolism; 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