{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:53:17Z","timestamp":1790553197273,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T00:00:00Z","timestamp":1529366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001808","name":"Kommission f\u00fcr Technologie und Innovation","doi-asserted-by":"publisher","award":["19086.1 PFLS\u2010LS"],"award-info":[{"award-number":["19086.1 PFLS\u2010LS"]}],"id":[{"id":"10.13039\/501100001808","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glucocorticoids are a mainstay for the treatment of immune\u2010mediated conditions and inflammatory diseases. However, their chronic use causes numerous side\u2010effects including delays in corneal and cutaneous wound healing. This is attributed to off\u2010target agonism of the mineralocorticoid receptor, which can be reduced by co\u2010administration of a mineralocorticoid receptor antagonist such as spironolactone. The aim of this study was to develop a fast, selective and sensitive UHPLC\u2010ESI\u2010MS method for the simultaneous quantification of spironolactone, its active metabolites (7<jats:italic>\u03b1<\/jats:italic>\u2010thiomethylspironolactone and canrenone), the latter's water\u2010soluble prodrug potassium canrenoate and the synthetic glucocorticoid, dexamethasone, in corneal samples (17<jats:italic>\u03b1<\/jats:italic>\u2010methyltestosterone served as an internal standard). A one\u2010step extraction procedure using MeOH\u2013H<jats:sub>2<\/jats:sub>O (1:1) was validated and employed to recover the analytes from the corneal tissue. Extracts were centrifuged and the supernatant analyzed under isocratic conditions. Compounds were detected using selected ion recording mode. The method satisfied US Food and Drug Administration guidelines with respect to selectivity, precision and accuracy and displayed linearity from 5 to 1000\u2009ng\/mL for all of the analytes. The lower limit of quantitation of the method was 5\u2009ng\/mL, making it sufficiently sensitive for quantification of the analytes in samples from <jats:italic>in vivo<\/jats:italic> studies.<\/jats:p>","DOI":"10.1002\/bmc.4287","type":"journal-article","created":{"date-parts":[[2018,5,16]],"date-time":"2018-05-16T07:04:23Z","timestamp":1526454263000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development and validation of a fast and sensitive UHPLC\u2010ESI\u2010MS method for the simultaneous quantification of spironolactone and its metabolites in ocular tissues"],"prefix":"10.1002","volume":"32","author":[{"given":"Naoual","family":"Dahmana","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Gabriel","sequence":"additional","affiliation":[{"name":"Apidel SA  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Gurny","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva University of Lausanne  Lausanne Switzerland"},{"name":"Apidel SA  Geneva Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-5489","authenticated-orcid":false,"given":"Yogeshvar N.","family":"Kalia","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences, University of Geneva University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.1997.00626.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0000000000000614"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S86778"},{"issue":"2","key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0090-9556(25)09198-6","article-title":"Re\u2010evaluation of the antimineralocorticoid effect of the spironolactone metabolite, canrenone, from plasma concentrations determined by a new high\u2010pressure liquid\u2010chromatographic method","volume":"7","author":"Dahlof C. 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