{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:37:50Z","timestamp":1784173070686,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T00:00:00Z","timestamp":1531699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two selective and accurate chromatographic methods are presented for simultaneous quantitation of spironolactone (SP) and furosemide (FR) and canrenone (CN), the main degradation product and the main active metabolite of SP. Method A was HPTLC, where separation was completed on silica gel HPTLC F<jats:sub>254<\/jats:sub> plates using ethyl acetate\u2013triethylamine\u2013acetic acid (9:0.7:0.5, by volume) as a developing system and UV detection at 254\u2009nm. Method B was a green isocratic RP\u2010HPLC utilizing a C<jats:sub>18<\/jats:sub> (4.6\u2009\u00d7\u2009100\u2009mm) column, the mobile phase consisting of ethanol\u2013deionized water (45: 55, <jats:italic>v<\/jats:italic>\/v) and UV estimation at 254\u2009nm. Adjustment of flow rate at 1\u2009mL\/min and pH at 3.5 with glacial acetic acid was done. Regarding the greenness profile, the proposed RP\u2010HPLC method is greener than the reported one. ICH guidelines were followed to validate the developed methods. Successful applications of the developed methods were revealed by simultaneous determination of FR, SP and CN in pure forms and plasma samples in the ranges of 0.2\u20132, 0.05\u20132.6 and 0.05\u20132\u2009\u03bcg\/band for method A and 5\u201360, 2\u201360 and 2\u201360\u2009\u03bcg\/mL for method B for FR, SP and CN, respectively.<\/jats:p>","DOI":"10.1002\/bmc.4304","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T01:41:46Z","timestamp":1527817306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma"],"prefix":"10.1002","volume":"32","author":[{"given":"Ibrahim A.","family":"Naguib","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-2490","authenticated-orcid":false,"given":"Eglal A.","family":"Abdelaleem","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aml A.","family":"Emam","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouruddin W.","family":"Ali","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1374-7907","authenticated-orcid":false,"given":"Fatma F.","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1556\/1006.2015.28.4.8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1981.tb00897.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2014.896901"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40508-016-0051-z"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/b617536h"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.farmac.2003.07.005"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1006"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10895-009-0479-6"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201401151"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2017.11.034"},{"key":"e_1_2_8_12_1","volume-title":"Ratio\u2010Spectra zero\u2010crossing derivative spectrophotometric determination of certain drugs in two\u2010component mixtures","author":"ERK, N","year":"2008"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1061934806080053"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(03)00422-9"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/chrsci\/49.2.129"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.11.055"},{"key":"e_1_2_8_17_1","first-page":"11","volume-title":"Validation of analytical procedures: Text and methodology Q2 (R1)","author":"ICH Harmonised Tripartite Guideline","year":"2005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2010.06.034"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr068359e"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1977.tb02146.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.farmac.2005.02.001"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.12.015"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.5958\/2394-2797.2016.00024.1"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bms062"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/00032710600824698"},{"issue":"1","key":"e_1_2_8_26_1","first-page":"34","article-title":"No TitFlow injection spectrophotometric determination of furosemide in pharmaceuticals by the bleaching of a permanganate carrier solutionle","volume":"22","author":"Semaan F. 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