{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:48:57Z","timestamp":1760060937292,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,8,19]],"date-time":"2018-08-19T00:00:00Z","timestamp":1534636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81503262"],"award-info":[{"award-number":["81503262"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tubeimoside I (Tub) is a triterpenoid saponin isolated from <jats:italic>Bolbostemma paniculatum[Maxim]Franquet<\/jats:italic>. A sensitive and validated method was developed to determine Tub in rat plasma. This method combined the qualitative and quantitative advantages from liquid chromatography coupled with hybrid ion trap\u2010time of flight mass spectrometer (HPLC\u2010DAD\u2010IT\u2010TOF\u2010MS) and a triple quadrupole\u2013linear ion trap mass spectrometer 5500 (Qtrap 5500), owing to the narrow molecular range of Qtrap 5500 relative to the molecular weight of Tub. Initially, ion detection was achieved using negative ionization mode along with full scan on IT\u2010TOF\u2010MS. The detected precursor and product ions of Tub with the optimal mass parameters were determined on Qtrap 5500 by an online stepped optimization strategy and operated in negative multiple reaction monitoring mode. A simple methanol precipitation was employed with saikosaponin A as internal standard. The method was validated over the range from 20 to 2000\u2009ng\/mL with a lower limit of quantification of 20\u2009ng\/mL for Tub in plasma. The developed method was successfully applied to the pharmacokinetic study of Tub in rats following oral administration. Moreover, this method has some directive significance for the determination of other drugs whose parent ions exceeding the upper detection limit in Qtrap 5500.<\/jats:p>","DOI":"10.1002\/bmc.4337","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T04:07:25Z","timestamp":1531454845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A hybrid method for the determination of tubeimoside I in rat plasma after oral administration by LC\u2010IT\u2010TOF and UPLC\u2010MS\/MS"],"prefix":"10.1002","volume":"32","author":[{"given":"Ying","family":"Li","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica, Modern Research Center for Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lifang","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Modern Research Center for Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianzhao","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Beijing University of Chinese Medicine Affiliated Dongzhimen Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongan","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Beijing University of Chinese Medicine Affiliated Dongzhimen Hospital  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7027-3002","authenticated-orcid":false,"given":"Pengfei","family":"Tu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Modern Research Center for Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinling","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Modern Research Center for Traditional Chinese Medicine Beijing University of Chinese Medicine  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,19]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"12517","article-title":"Tubeimoside\u20101 inhibits the growth and invasion of colorectal cancer cells through the Wnt\/beta\u2010catenin signaling pathway","volume":"8","author":"Bian Q.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2016.08.028"},{"key":"e_1_2_6_4_1","first-page":"535","article-title":"Tubeimoside\u20101 induces G2\/M phase arrest and apoptosis in SKOV\u20103 cells through increase of intracellular Ca(2)(+) and caspase\u2010dependent signaling pathways","volume":"40","author":"Chen W. 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