{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T06:28:15Z","timestamp":1782973695599,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,10,7]],"date-time":"2018-10-07T00:00:00Z","timestamp":1538870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["BMS\/FW\/Nano\/2015\u201020580\/March15\/14\/govt"],"award-info":[{"award-number":["BMS\/FW\/Nano\/2015\u201020580\/March15\/14\/govt"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methotrexate (MTX) and teriflunomide (TEF) are the two most effective disease\u2010modifying antirheumatic drugs used as combination therapy for rheumatoid arthritis and no robust high\u2010performance liquid chromatography (HPLC) method is available for their simultaneous estimation to date. Therefore, we have developed and validated an isocratic reversed\u2010phase HPLC method for simultaneous analysis of MTX and TEF spiked in the form of active pharmaceutical ingredients, tablets and nanoformulations. The best separation was achieved on a BDS, C<jats:sub>18<\/jats:sub>, 4.6\u2009\u00d7\u2009250\u2009mm, 5\u2009\u03bcm analytical column (Thermo Hypersil) with methanol\u2013ethylammonium formate\u2013potassium dihydrogen phosphate buffer (55\u2009m<jats:sc>m<\/jats:sc>, pH\u20093.5; 65:5:30, v\/v) as mobile phase at a flow rate of 0.8\u2009mL\/min. All the samples were subjected to force degradation studies. Responses of MTX and TEF were found to be a linear function of concentration over the range 1\u201350\u2009\u03bcg\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.9976 and 0.9982). The limits of detection and limit of quantification were 7.74 and 25.82\u2009ng\/mL and 10.74 and 35.80\u2009ng\/mL, respectively. Degradation products produced under the stress studies did not interfere with the detection of MTX and TEF and therefore the developed method can be regarded as stability indicating.<\/jats:p>","DOI":"10.1002\/bmc.4372","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T15:51:26Z","timestamp":1534953086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Development and validation of stability indicating reversed\u2010phase liquid chromatographic method for simultaneous quantification of methotrexate and teriflunomide in nanoparticles and marketed formulation"],"prefix":"10.1002","volume":"32","author":[{"given":"Shweta","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asiya","family":"Mahtab","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archu","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhan Jalees","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd.","family":"Aqil","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5301-5298","authenticated-orcid":false,"given":"Sushama","family":"Talegaonkar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, School of Pharmaceutical Education and Research Jamia Hamdard  New Delhi India"},{"name":"Department of Pharmaceutics Delhi Pharmaceutical Sciences &amp; Research University  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.10.035"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1556\/AChrom.20.2008.1.8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2013.09.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2015.1087409"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.12.034"},{"issue":"3","key":"e_1_2_9_7_1","first-page":"168","article-title":"Understanding the mechanisms of action of methotrexate implications for the treatment of rheumatoid arthritis","volume":"65","author":"Henghe T.","year":"2007","journal-title":"Bulletin of the NYU Hospital for Joint Diseases"},{"key":"e_1_2_9_8_1","volume-title":"Test procedures and acceptance criteria for new drug substances and new drug products: Chemical substances. 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J.","year":"2011","journal-title":"International Journal of ChemTech Research"},{"issue":"5","key":"e_1_2_9_12_1","first-page":"12","article-title":"A validated stability indicating reversed phase high performance liquid chromatographic method of leflunomide and characterization of its degradation products through retro\u2010synthesis","volume":"7","author":"Laha T. K.","year":"2017","journal-title":"Journal of Applied Pharmaceutical Science"},{"issue":"3","key":"e_1_2_9_13_1","first-page":"443","article-title":"Development and validation of RP\u2010HPLC method for simultaneous determination of methotrexate, dexamethasone and indomethacin","volume":"7","author":"Lariya N. 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