{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T19:05:56Z","timestamp":1776452756571,"version":"3.51.2"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T00:00:00Z","timestamp":1540857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fred & Pamela Buffett Cancer Center Support Grant from the National Cancer Institute","award":["P30 CA036727 and F31 CA224942-01A1"],"award-info":[{"award-number":["P30 CA036727 and F31 CA224942-01A1"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Moxidectin (MOX) has recently been approved by the US Food and Drug Administration for the treatment of river blindness in select populations. It is also being evaluated as an alternative for the use of ivermectin, widespread resistance to which is becoming a global health issue. Moreover, MOX is becoming increasingly used as a prophylactic antiparasitic in the cattle industry. In this study, we developed and validated an LC\u2013MS\/MS method of MOX in human, monkey and mouse plasma. The separation was achieved on an ACE C<jats:sub>18<\/jats:sub> (50\u2009\u00d7 3.0\u2009mm, 3\u2009\u03bcm) column with isocratic elution using 0.1% acetic acid and methanol\u2013acetonitrile (1:1, v\/v) as mobile phase. MOX was quantitated using MS\/MS with an electrospray ionization source operating in negative multiple reaction monitoring mode. The multiple reaction monitoring precursor ion \u2192 product ion transitions for MOX and abamectin (IS) were <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 638.40\u00a0\u2192\u00a0236.30 and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 871.50\u00a0\u2192\u00a0565.35 respectively. The MS\/MS response was linear over the concentration range 0.1\u20131000\u2009ng\/mL in plasma with a correlation coefficient (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>) of 0.997 or better. The within\u2010 and between\u2010day precision (relative standard deviation, RSD) and accuracy were within the acceptable limits per US Food and Drug Administration guidelines. The method was successfully applied to an <jats:italic>in vitro<\/jats:italic> metabolic stability study of MOX.<\/jats:p>","DOI":"10.1002\/bmc.4389","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T12:30:05Z","timestamp":1537533005000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Bioanalytical method development and validation of moxidectin in plasma by LC\u2013MS\/MS: Application to <i>in vitro<\/i> metabolism"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6455-5388","authenticated-orcid":false,"given":"Yashpal S.","family":"Chhonker","sequence":"first","affiliation":[{"name":"Department of Pharmacy Practice University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard L.","family":"Sleightholm","sequence":"additional","affiliation":[{"name":"Center for Drug Delivery and Nanomedicine, Department of Pharmaceutical Sciences University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-5027","authenticated-orcid":false,"given":"Daryl J.","family":"Murry","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice University of Nebraska Medical Center  Omaha NE USA"},{"name":"Fred and Pamela Buffett Cancer Center University of Nebraska Medical Center  Omaha NE USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,30]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/jaoac\/83.1.31","article-title":"Simultaneous determination of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin in beef liver by LC with fluorescence detection","volume":"83","author":"Ali M. 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