{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:33:13Z","timestamp":1775061193728,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T00:00:00Z","timestamp":1104364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A method was developed for the quantitation of pentoxifylline [1\u2010(5\u2010oxohexyl)\u20103,7\u2010dimethylxanthine] and a primary active metabolite, lisofylline [1\u2010(5\u2010hydroxyhexyl)\u20103,7\u2010dimethylxanthine], using high\u2010performance liquid chromatography (HPLC)\u2013tandem mass spectrometry. This method was developed in order to overcome problems encountered with HPLC\u2013ultraviolet detection. The operating parameters of the electrospray interface (PE SCIEX, TurboIon Spray) and lens voltages of the triple\u2010quadrupole detector (PE SCIEX 365) were optimized in positive ion mode to obtain the best sensitivity of the analytes. Collision\u2010induced dissociation was used to produce fragment ions, and multiple reaction monitoring was used to quantitate pentoxifylline (<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 279\/181) and lisofylline (<jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 263\/181). Dichloromethane was used to extract the drug, metabolite, and the internal standard (3\u2010isobutyl\u20101\u2010methylxanthine) from plasma. A reverse\u2010phase C8(2) 150 \u00d7 1.0 mm HPLC column was used to resolve all three compounds in less than 6 min. Calibration curves were generated using peak area and were linear from 1 to 1000 ng\/mL (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.99). The small sample volume, ease of extraction, and sensitivity provide advantages over more conventional methods of quantitation. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.441","type":"journal-article","created":{"date-parts":[[2004,12,30]],"date-time":"2004-12-30T09:24:45Z","timestamp":1104398685000},"page":"231-236","source":"Crossref","is-referenced-by-count":12,"title":["Use of liquid chromatography\u2013tandem mass spectrometry for the analysis of pentoxifylline and lisofylline in plasma"],"prefix":"10.1002","volume":"19","author":[{"given":"Patrick B.","family":"Kyle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim G.","family":"Adcock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Kramer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodney C.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.09.019"},{"key":"e_1_2_1_3_1","volume-title":"Disposition of Toxic Drugs and Chemicals in Man","author":"Baselt RC","year":"2002"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80336-5"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)80271-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80302-X"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(02)00356-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.11.019"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac010514w"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(92)80509-O"},{"key":"e_1_2_1_11_1","first-page":"209","article-title":"Hemorheology and peripheral vascular diseases: a new therapeutic approach","volume":"12","author":"Muller R","year":"1981","journal-title":"Journal of Medicine"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.288"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/b100076o"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(00)00189-6"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600750111"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365510308569"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(01)00255-0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2003.140"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990815)13:15<1611::AID-RCM683>3.0.CO;2-C"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00398-7"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.441","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T20:24:23Z","timestamp":1760041463000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12,30]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1002\/bmc.441"],"URL":"https:\/\/doi.org\/10.1002\/bmc.441","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12,30]]}}}