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The analysis required extraction of a 200\u2009\u03bcL plasma sample and precipitation of proteins using solid\u2010phase extraction. Ibrutinib and nilotinib, which was used as an internal standard, were separated using high\u2010performance liquid chromatography (HPLC) using a mobile phase of acetonitrile\u20130.5% monopotassium phosphate (KH<jats:sub>2<\/jats:sub>PO<jats:sub>4<\/jats:sub>, pH\u20093.0; 52:48, v\/v) on a Capcell Pack C<jats:sub>18<\/jats:sub> MG II (250\u2009\u00d7\u20094.6\u2009mm) monitored at 260\u2009nm, at a flow rate of 1.0\u2009mL\/min. The calibration curve was linear at the plasma concentration range of 10\u2013500\u2009ng\/mL with a coefficient of determination (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>) of 0.9999. The coefficients of intra\u2010day and inter\u2010day validation were 4.0\u20136.6 and 2.6\u20137.7%, respectively. The assay accuracy was \u22124.4\u20138.6%, and the recovery was &gt;84%. This HPLC method coupled with ultraviolet (UV) detection for determining ibrutinib plasma concentration has several advantages such as simplicity and applicability to routine therapeutic drug monitoring at hospital laboratories.<\/jats:p>","DOI":"10.1002\/bmc.4435","type":"journal-article","created":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T07:54:51Z","timestamp":1542095691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Simple determination of plasma ibrutinib concentration using high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0587-0091","authenticated-orcid":false,"given":"Takeo","family":"Yasu","sequence":"first","affiliation":[{"name":"Department of Pharmacy, The Research Hospital The Institute of Medical Science, The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Momo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Research Hospital The Institute of Medical Science, The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yasui","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology The Institute of Medical Science, The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichirou","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, The Research Hospital The Institute of Medical Science, The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.42.7906"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-06-722991"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-12-737346"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1509388"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1400376"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)00465-9"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-2225"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-015-2708-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpt.419"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1802917"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00667-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.147900"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199630030-00001"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-11-818286"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000357"},{"key":"e_1_2_7_17_1","unstructured":"Imbruvica\u00ae. 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