{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T20:05:01Z","timestamp":1769889901309,"version":"3.49.0"},"reference-count":8,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Valproic acid (VPA) pharmacokinetics is highly variable and monitoring of blood levels is necessary to determine its appropriate dosage. This study aimed to establish and validate a novel derivatization method for the determination of VPA. The method was based on the catalytic effect of tetramethylammonium hydroxide using 2,4\u2032\u2010dibromoacetophenone as a derivatization reagent. After derivatization, samples were injected into the HPLC system for analysis. The method showed a good linearity in the range of 1.0\u2013200.7\u2009\u03bcg mL<jats:sup>\u22121<\/jats:sup>, and the limit of quantification was 1\u2009\u03bcg mL<jats:sup>\u22121<\/jats:sup>. All values of the accuracy and relative standard deviations were acceptable for the analyses of biological samples. The recoveries were in the range from 91.6 to 97.4% for VPA with RSD &lt;3.9%. A novel and high conversion\u2010rate derivatization method has been developed and validated for the determination of VPA in human serum. It can be applied to the analysis of VPA in clinic serum samples.<\/jats:p>","DOI":"10.1002\/bmc.4440","type":"journal-article","created":{"date-parts":[[2018,11,20]],"date-time":"2018-11-20T02:02:07Z","timestamp":1542679327000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A new derivatization method for the determination of valproic acid in serum using tetramethylammonium hydroxide as a catalyst"],"prefix":"10.1002","volume":"33","author":[{"given":"Guifu","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Pharmacy Liuzhou Traditional Chinese Medical Hospital  Guangxi People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Beijing Friendship Hospital Pinggu Campus, Capital Medical University  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shusen","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Health Sciences Western New England University  Springfield Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanxia","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The General Hospital of the Chinese People's Armed Police Forces  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6726-9571","authenticated-orcid":false,"given":"Xingang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Beijing Tiantan Hospital, Capital Medical University  Beijing People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,13]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-40422009000500027"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(83)80010-2"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(84)80115-2"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-0254-8"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015709126921"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.inorgchem.6b03131"},{"key":"e_1_2_4_8_1","first-page":"355","article-title":"New RP\u2010HPLC method for the determination of valproic acid in human plasma","volume":"2","author":"Venkata Nagendra Prasad C.","year":"2010","journal-title":"Journal of Pharmaceutical Sciences and Research"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmt167"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4440","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4440","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.4440","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:59:45Z","timestamp":1760039985000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,13]]},"references-count":8,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/bmc.4440"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4440","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,13]]},"assertion":[{"value":"2018-07-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4440"}}