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Samples were extracted by deproteinization with acetonitrile, and the extracts were cleaned up by strong anion exchange and weak cation exchange applied sequentially. The substances were detected by rapid liquid chromatography tandem mass spectrometry. Matrix components were largely removed by the clean\u2010up, resulting in low matrix effects. The lower limits of quantification for an extracted tissue mass of 100\u2009mg were 0.3, 0.3, 0.2 and 2\u2009ng\/g for DA, 5\u2010HT, 5\u2010HIAA and DOPAC, respectively. The mean true extraction recoveries were 80\u2013102%. The relative intra\u2010laboratory reproducibility standard deviations were generally &lt;11% at concentrations of 20\u20131000\u2009ng\/g for DA, 5\u2010HT, 5\u2010HIAA and DOPAC and 7% at concentrations of 5\u201350\u2009\u03bcg\/g for the amino acids. This method was successfully used in a phenylketonuria mice study including nearly 300 brain tissue samples and for small sample masses (for example, 2\u2009mg of bulbus olfactorius).<\/jats:p>","DOI":"10.1002\/bmc.4479","type":"journal-article","created":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T17:12:01Z","timestamp":1546276321000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Sensitive determination of monoamine neurotransmitters, their main metabolites and precursor amino acids in different mouse brain components by liquid chromatography\u2013electrospray tandem mass spectrometry after selective sample clean\u2010up"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4262-0035","authenticated-orcid":false,"given":"Lambert K.","family":"S\u00f8rensen","sequence":"first","affiliation":[{"name":"Section for Forensic Chemistry, Department of Forensic Medicine Aarhus University  Aarhus N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mogens","family":"Johannsen","sequence":"additional","affiliation":[{"name":"Section for Forensic Chemistry, Department of Forensic Medicine Aarhus University  Aarhus N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60961-0"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.07.043"},{"key":"e_1_2_5_4_1","unstructured":"European Medicines Agency(2011).Guideline on bioanalytical method validation. 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