{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T23:25:45Z","timestamp":1767828345522,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:00:00Z","timestamp":1550448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Food and Drug Safety Administration, Republic of Korea","award":["16162MFDS582"],"award-info":[{"award-number":["16162MFDS582"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pesticides, which are used as plant protection products, can enter the food chain, and exposure to these xenobiotics can cause a wide array of health problems in humans. Therefore, the objective of the present study was to develop an analytical method for the simultaneous determination of residual spinosad (sum of spinosyn A and D), temephos and piperonyl butoxide in porcine muscle, egg, milk, eel, flatfish and shrimp (sampling period: February to June 2018) using liquid chromatography\u2013triple quadrupole tandem mass spectrometry (LC\u2013MS\/MS). The target analytes were extracted with a combination of acidified acetonitrile and ethyl acetate and subsequently purified with original QuEChERS kits (composed of magnesium sulfate and sodium chloride) as well as <jats:italic>n<\/jats:italic>\u2010hexane. All analytes were separated on a reversed\u2010phase analytical column using a mobile phase of (A) 0.1% formic acid containing 10\u2009m<jats:sc>m<\/jats:sc> ammonium formate in distilled water and (B) methanol. Good linearity (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009\u2265\u20090.980) was achieved over the tested concentration range (3.5\u201335\u2009\u03bcg\/kg for spinosyn A; 1.5\u201315\u2009\u03bcg\/kg for spinosyn D; 5\u201350\u2009\u03bcg\/kg for temephos and piperonyl butoxide) in matrix\u2010matched standard calibrations. Fortified samples at three spiking levels yielded recoveries in the range of 71\u2013105% with relative standard deviations \u22649.2%. The applicability of the method was evaluated via evaluating samples collected from a large wholesale market located in Seoul, and none of the samples contained any of the target analytes. In conclusion, the current approach is simple, efficient and reliable and can successfully determine the residual levels of spinosad, temephos and piperonyl butoxide in complex animal\u2010derived food products.<\/jats:p>","DOI":"10.1002\/bmc.4493","type":"journal-article","created":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T04:23:04Z","timestamp":1548044584000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Simultaneous determination of spinosad, temephos, and piperonyl butoxide in animal\u2010derived foods using LC\u2013MS\/MS"],"prefix":"10.1002","volume":"33","author":[{"given":"Weijia","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010Min","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7907","authenticated-orcid":false,"given":"A. M.","family":"Abd El\u2010Aty","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Veterinary Medicine Cairo University  Giza Egypt"},{"name":"Department of Medical Pharmacology, Medical Faculty Ataturk University  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung\u2010Hee","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Hee","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong\u2010Kwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young\u2010Sun","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"},{"name":"Department of Biomedical Science and Technology Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Hac\u0131m\u00fcft\u00fco\u011flu","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology, Medical Faculty Ataturk University  Erzurum Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro\u2010Product Quality and Safety Institute of Quality Standard and Testing Technology for Agro\u2010Product, Chinese Academy of Agricultural Sciences  Beijing PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5361-2903","authenticated-orcid":false,"given":"Jae\u2010Han","family":"Shim","sequence":"additional","affiliation":[{"name":"Natural Products Chemistry Laboratory, College of Agriculture and Life Sciences Chonnam National University  Gwangju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5500-3901","authenticated-orcid":false,"given":"Ho\u2010Chul","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine Konkuk University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,18]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Codex Alimentarius Commission(1993).Codex Guidelines for the Establishment of a Regulatory Programme for Control of Veterinary Drug Residues in Foods. 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