{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:35Z","timestamp":1786977275429,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T00:00:00Z","timestamp":1551312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin are four fluoroquinolones used in the treatment of serious bacterial infections. The antibacterial activity of fluoroquinolones is concentration dependent. Therefore, therapeutic drug monitoring in daily clinical practice is warranted to ensure the therapy's efficacy and prevent bacterial resistance. The purpose of the present study was to develop a method using high\u2010pressure liquid chromatography with an ultraviolet detector for simultaneous quantification of these four fluoroquinolones in human plasma. A 50\u2009\u03bcL aliquot of plasma was precipitated by 200\u2009\u03bcL of methanol using gatifloxacin as internal standard. The chromatographic separation was performed on a Kinetex XB\u2010C\n                    <jats:sub>18<\/jats:sub>\n                    column using a mobile phase composed of a mixture of orthophosphoric acid 0.4% (v\/v), acetonitrile and methanol at a flow rate of 1.2\u2009mL\/min. Dual UV wavelength mode was used, with levofloxacin and moxifloxacin monitored at 293\u2009nm, and pefloxacin and ciprofloxacin monitored at 280\u2009nm. The calibration was linear over the ranges of 0.125\u201325\u2009mg\/L for levofloxacin, 0.1\u201320mg\/L for moxifloxacin and 0.05\u201010\u2009mg\/L for both pefloxacin and ciprofloxacin. Inter\u2010 and intra\u2010day trueness and precision were &lt;13% for all the compounds under study. The proposed method was simple, reliable, cost\u2010effective and suitable for therapeutic drug monitoring or pharmacokinetics studies.\n                  <\/jats:p>","DOI":"10.1002\/bmc.4506","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T18:58:56Z","timestamp":1549911536000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in microvolumes of human plasma using high\u2010performance liquid chromatography with ultraviolet detection"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1115-7121","authenticated-orcid":false,"given":"Yi","family":"Zheng","sequence":"first","affiliation":[{"name":"Service de Pharmacologie Clinique, H\u00f4pital Cochin, Assistance Publique des H\u00f4pitaux de Paris Groupe Hospitalier Paris Centre  Paris France"},{"name":"EA 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