{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:13:46Z","timestamp":1792451626328,"version":"4.5.0"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T00:00:00Z","timestamp":1551830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences","doi-asserted-by":"crossref","award":["2018C005"],"award-info":[{"award-number":["2018C005"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tricaine methanesulfonate is one of most commonly used anesthetics in fish during blood sampling, artificial propagation and long\u2010distance transportation. In this study, an accurate method for the quantitative determination of tricaine in fish samples by a stable isotope dilution assay coupled with high\u2010performance liquid chromatography\u2013triple quadrupole mass spectrometry was developed. Tricaine\u2010D<jats:sub>5<\/jats:sub> was synthesized and used as an isotopically labeled internal standard for the determination of tricaine. The analytical performance of the method was validated for tricaine determination in marine fish and freshwater fish. The determination of tricaine was linear in the range of 2.0\u2013200.0\u2009\u03bcg\u2009L<jats:sup>\u22121<\/jats:sup>. The limit of detection and limit of quantitation for fish muscle tissues were 1.0\u2009and 4.0\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup>, respectively. Good recoveries were obtained in the range of 92.08\u201397.50%. The inter\u2010 and intra\u2010assay relative standard deviations (RSD values) were investigated, and the values were 0.39\u20133.01 and 0.85\u20132.77%, respectively. The values of CC<jats:italic>\u03b1<\/jats:italic> and CC<jats:italic>\u03b2<\/jats:italic> were 10.21\u201310.43 and 10.42\u201310.87\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup>, respectively. The clearance of MS\u2010222 from grass carp was further studied using our method. The results demonstrate that MS\u2010222 could be well absorbed and rapidly eliminated after bath administration.<\/jats:p>","DOI":"10.1002\/bmc.4512","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T02:21:01Z","timestamp":1550802061000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Stable isotope dilution assay for the accurate determination of tricaine in fish samples by HPLC\u2013MS\u2013MS"],"prefix":"10.1002","volume":"33","author":[{"given":"Chaonan","family":"Xie","sequence":"first","affiliation":[{"name":"Chinese Academy of Fishery Sciences  Beijing P. R. China"},{"name":"Key Laboratory of Control of Quality and Safety for Aquatic Products Ministry of Agriculture  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences  Beijing P. R. China"},{"name":"Key Laboratory of Control of Quality and Safety for Aquatic Products Ministry of Agriculture  Beijing P. R. China"},{"name":"College of Fisheries and Life Science Shanghai Ocean University  Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Han","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences  Beijing P. R. China"},{"name":"Key Laboratory of Control of Quality and Safety for Aquatic Products Ministry of Agriculture  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences  Beijing P. R. China"},{"name":"Key Laboratory of Control of Quality and Safety for Aquatic Products Ministry of Agriculture  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jien","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Physics and Materials Science Henan Normal University  Xinxiang P. R. China"},{"name":"Henan Key Laboratory of Photovoltaic Materials  Xinxiang P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8363-3916","authenticated-orcid":false,"given":"Jincheng","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Fishery Sciences  Beijing P. R. China"},{"name":"Key Laboratory of Control of Quality and Safety for Aquatic Products Ministry of Agriculture  Beijing P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2885.1997.00094.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11160-010-9188-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.10.026"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.06.054"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaeng.2017.02.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10499-014-9751-0"},{"key":"e_1_2_6_8_1","unstructured":"Health Canada(2013).Proposal to amend the List of Maximum Residue Limits (MRLs) for veterinary drugs in foods\u2014Proposed MRL 2013\u20131. Retrieved fromhttps:\/\/www.canada.ca\/en\/health\u2010canada\/services\/drugs\u2010health\u2010products\/veterinary\u2010drugs\/maximum\u2010residue\u2010limits\u2010mrls\/list\u2010maximum\u2010residue\u2010limits\u2010mrls\u2010veterinary\u2010drugs\u2010foods.html. (Accessed 20 May 2018)"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-4039(96)01351-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.11.112"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AY00784K"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.8867"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2017.01.027"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2006.11.008"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12562-017-1091-3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201600614"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.4512","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:13:32Z","timestamp":1760055212000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,6]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1002\/bmc.4512"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4512","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,6]]},"assertion":[{"value":"2018-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4512"}}