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The potential of sphingosine 1\u2010phosphate (S1P) as a plasma marker in human diseases was suggested recently in the literature, which demonstrated that, in patients with inflammatory diseases, plasma S1P was elevated. It follows that the obstructive coronary artery disease can be predicted with serum S1P. Therefore, S1P can also be potentially used as a pharmacodynamic marker to study adjuvant arthritis (AA) rats. In the current study, a UHPLC\u2013MS\/MS method combined with the microdialysis sampling technique (using FTY720 phosphate as an internal standard) was adopted and validated to measure S1P levels in the hemodialysis fluid and joint cavity dialysates of AA rats after oral administration of GE. A S1P concentration\u2013time curve in the dialysate was established in this study. It was demonstrated that GE exerted an anti\u2010inflammatory effect by reducing AA\u2010induced elevated S1P levels. It is showed that changes in S1P concentrations over time can be used to monitor the pharmacodynamic effects of GE in treating AA rats in pharmacodynamic studies.<\/jats:p>","DOI":"10.1002\/bmc.4526","type":"journal-article","created":{"date-parts":[[2019,3,4]],"date-time":"2019-03-04T21:27:57Z","timestamp":1551734877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["UHPLC\u2013MS\/MS analysis of sphingosine 1\u2010phosphate in joint cavity dialysate and hemodialysis solution of adjuvant arthritis rats: Application to geniposide pharmacodynamic study"],"prefix":"10.1002","volume":"33","author":[{"given":"Dai","family":"Xuejing","sequence":"first","affiliation":[{"name":"Anhui University of Chinese Medicine  Hefei China"},{"name":"Anhui Province Key Laboratory of Xin'an Medicine, Ministry of Education  Hefei China"},{"name":"Anhui Province Key Laboratory of Chinese Medicinal Formula  Hefei 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