{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T01:27:39Z","timestamp":1780622859755,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tolnaftate, a thionoester anti\u2010fungal drug, was subjected to alkaline hydrolysis to produce methyl(<jats:italic>m<\/jats:italic>\u2010tolyl)carbamic acid and <jats:styled-content><jats:italic>\u03b2<\/jats:italic><\/jats:styled-content>\u2010naphthol (tolnaftate impurity A). <jats:italic>N<\/jats:italic>\u2010Methyl\u2010<jats:italic>m<\/jats:italic>\u2010toluidine, tolnaftate impurity D, was synthesized and structurally elucidated along with tolnaftate alkaline degradation products using IR, H<jats:sup>1<\/jats:sup>NMR and MS. Two stability\u2010indicating HPTLC and RP\u2010HPLC methods were developed and validated, for the first time, for determination of tolnaftate, its alkaline degradation products and toxic impurities in the presence of methyl paraben, as a preservative in Tinea Cure<jats:sup>\u00ae<\/jats:sup> cream. The proposed HPTLC method depended on separation of the studied components on TLC silica gel F<jats:sub>254<\/jats:sub> plates using hexane\u2013glacial acetic acid (8:2, v\/v) as a developing system and scanning wavelength of 230\u2009nm. The proposed RP\u2010HPLC method was based on separation of the five components on an Eclipse plus C<jats:sub>18<\/jats:sub> column. The mobile phase used was acetonitrile\u2013water containing 1% ammonium formate (40:60, v\/v), with a flow rate of 1\u2009mL\/min and detection wavelength of 230\u2009nm. The proposed methods allowed the assay of tolnaftate toxic impurities, <jats:styled-content><jats:italic>\u03b2<\/jats:italic><\/jats:styled-content>\u2010naphthol and <jats:italic>N<\/jats:italic>\u2010methyl\u2010<jats:italic>m<\/jats:italic>\u2010toluidine, down to 2%, allowing tracing of <jats:styled-content><jats:italic>\u03b2<\/jats:italic><\/jats:styled-content>\u2010naphthol that could be absorbed by the skin causing systemic toxic effects, unlike tolnaftate, indicating the high significance of such determination. International Conference on Harmonization guidelines were followed for validation.<\/jats:p>","DOI":"10.1002\/bmc.4547","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T23:33:51Z","timestamp":1554248031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Two validated chromatographic determinations of an antifungal drug, its toxic impurities and degradation product: A comparative study"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-2490","authenticated-orcid":false,"given":"Eglal A.","family":"Abdelaleem","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Al shaheed Shehata Ahmed Hegazy st. 62514 Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0700-7542","authenticated-orcid":false,"given":"Maha M.","family":"Abdelrahman","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Al shaheed Shehata Ahmed Hegazy st. 62514 Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouruddin W.","family":"Ali","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Al shaheed Shehata Ahmed Hegazy st. 62514 Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6526-4419","authenticated-orcid":false,"given":"Raghda A.","family":"Emam","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Beni\u2010Suef University  Al shaheed Shehata Ahmed Hegazy st. 62514 Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"17","article-title":"Development and validation of analytical method for simultaneous estimation of betamethasone dipropionate, tolnaftate, iodochlorhydroxyquin in pharmaceutical dosage form","volume":"2","author":"Bapna M.","year":"2015","journal-title":"The Pharmaceutical and Chemical Journal"},{"key":"e_1_2_7_3_1","unstructured":"Benger B.(1991).Proceedings of the sixth international symposium on instrumental planar chromatograpy. 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