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We developed and validated a bioanalytical method to detect MDZ in plasma using high\u2010performance liquid chromatography (HPLC) coupled to a photodiode array detector (PDA) for future monitoring of sedation. MDZ was extracted by solid\u2010phase extraction (SPE). Analyses were performed on a C<jats:sub>18<\/jats:sub> column, using 0.05% triethylamine and acetonitrile as mobile phase, analyzing at 220\u2009nm. Recovery was evaluated by comparing extracted and nonextracted solutions. Precision, accuracy, linearity, limits of detection (LD) and quantification (LQ), specificity and selectivity were determined. The mean recovery obtained by SPE was 101.03%. The method was linear in the range 1.0\u201350.0\u2009\u03bcg\/mL. The LD and LQ were, respectively, 0.43 and 1.43\u2009\u03bcg\/mL. The specificity of the MDZ peak was adequate. The method was able to detect MDZ among other drugs. Plasma anticoagulants showed no interference with the drug detection. The bioanalytical method using HPLC\u2013PDA and SPE was successfully validated and showed linearity, precision, accuracy, specificity and high sensitivity for detection of MDZ in human plasma.<\/jats:p>","DOI":"10.1002\/bmc.4564","type":"journal-article","created":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T23:35:19Z","timestamp":1556667319000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bioanalytical method to detect midazolam in plasma"],"prefix":"10.1002","volume":"33","author":[{"given":"B\u00e1dila Regina","family":"Dalla Costa","sequence":"first","affiliation":[{"name":"Curso de Farm\u00e1cia Universidade de Passo Fundo  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Anzolin","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Envelhecimento Humano Universidade de Passo Fundo  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana Grazziotin","family":"Rossato\u2010Grando","sequence":"additional","affiliation":[{"name":"Curso de Farm\u00e1cia Universidade de Passo Fundo  Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioexperimenta\u00e7\u00e3o Universidade de Passo Fundo  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7596-315X","authenticated-orcid":false,"given":"Charise Dallazem","family":"Bertol","sequence":"additional","affiliation":[{"name":"Curso de Farm\u00e1cia Universidade de Passo Fundo  Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,17]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Brasil Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria(2012).Resolu\u00e7\u00e3o da Diretoria Colegiada\u2014RDC no. 24 de 17 de Maio de 2012. Disp\u00f5e sobre os requisitos m\u00ednimos para a valida\u00e7\u00e3o de m\u00e9todos bioanal\u00edticos empregados em estudos com fins de registro e p\u00f3s\u2010registro. Brasil Minist\u00e9rio da Sa\u00fade."},{"key":"e_1_2_5_3_1","unstructured":"Brasil Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria(2017).Resolu\u00e7\u00e3o da Diretoria Colegiada\u2014RDC no. 166 de 24 de Julho de 2017. Disp\u00f5e sobre a valida\u00e7\u00e3o de m\u00e9todos anal\u00edticos e d\u00e1 outras provid\u00eancias. Brasil Minist\u00e9rio da Sa\u00fade."},{"volume-title":"As Bases Farmacol\u00f3gicas da Terap\u00eautica de Goodman e Guilman","year":"2012","author":"Brunton L. L.","key":"e_1_2_5_4_1"},{"key":"e_1_2_5_5_1","unstructured":"Chemicalize.Calculation. Retrieved fromhttps:\/\/chemicalize.com. Accessed at 03 Mar2017."},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-4679(97)00022-X"},{"key":"e_1_2_5_7_1","unstructured":"Dailymed(2017).All drugs. 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