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Owing to the instability of bupropion in serum, the main and active metabolite hydroxybupropion was chosen as the target substance. Therefore, a simple and robust high\u2010performance liquid chromatography method for the quantification of hydroxybupropion in serum was developed and validated. A volume of 30\u2009\u03bcL serum was used for easy sample clean\u2010up, based on protein precipitation with acetonitrile followed by online solid\u2010phase extraction. As hydroxybupropion was present in high serum concentrations, UV detection was possible. Owing to the commonly available instrumentation, the method could easily be integrated in routine TDM. The newly developed method was validated following the guidelines for bioanalytical method validation of the European Medicines Agency and US Food and Drug Administration. The lower limit of quantification was 100\u2009ng\/mL (0.391\u2009\u03bc<jats:sc>m<\/jats:sc>) and linearity was shown between 100 and 2500\u2009ng\/mL. Intraday and interday precision ranged from 1.17 to 6.79% and from 6.07 to 9.41%, respectively. Intraday and interday accuracy ranged from 89.97 to 110.86% and from 95.05 to 101.2%. The method was shown to be selective, accurate and precise. Additionally, the method was successfully implemented in the therapeutic drug monitoring laboratory of the Department of Psychiatry, Psychosomatics and Psychotherapy at the University Hospital of W\u00fcrzburg, Germany. Six months of routine analysis showed a rather low correlation between applied dose and serum concentration and therefore the necessity of TDM for dose\u2010individualization in the treatment with bupropion.<\/jats:p>","DOI":"10.1002\/bmc.4609","type":"journal-article","created":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T10:54:11Z","timestamp":1559300051000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Determination of hydroxybupropion in human serum for routine therapeutic drug monitoring in psychiatry: A tool for dose\u2010individualization in treatment with bupropion"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4780-612X","authenticated-orcid":false,"given":"Maike","family":"Scherf\u2010Clavel","sequence":"first","affiliation":[{"name":"Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Wahl","sequence":"additional","affiliation":[{"name":"Institute for Pharmacy and Food Chemistry University of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Menke","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Unterecker","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health University Hospital of W\u00fcrzburg  W\u00fcrzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.11.016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-832687"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1097\/FTD.0b013e31822fbf7c","article-title":"Therapeutic drug monitoring for drugs used in the treatment of substance\u2010related disorders: Literature review using a therapeutic drug monitoring appropriateness rating scale","volume":"33","author":"Brunen S.","year":"2011","journal-title":"Therapeutic Drug Monitoring"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-420118-7.00005-6"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.03.049"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.07.007"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1284425"},{"key":"e_1_2_9_9_1","unstructured":"EMA. 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