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Fries ex Nym (<jats:styled-content style=\"fixed-case\"><jats:italic>L. rotatum<\/jats:italic><\/jats:styled-content>), a member of Gentianaceae, is an important mongolian medicine in China used to treat febrile diseases in liver and gallbladder. The aim of present study was to investigate the chemical constituents and metabolites of the 50% ethanol fraction of <jats:styled-content style=\"fixed-case\"><jats:italic>L. rotatum<\/jats:italic><\/jats:styled-content> (50EtLR). Firstly, the extract of <jats:styled-content style=\"fixed-case\"><jats:italic>L. rotatum<\/jats:italic><\/jats:styled-content> was partitioned by macroporous resin to obtain the target fraction (50EtLR), then several compounds were isolated from 50EtLR to obtained the standards for further analysis of chemical constituents of 50EtLR. Secondly, the chemical constituents of 50EtLR were characterized using the ultra\u2010high performance liquid chromatography coupled with quadrupole\u2013time\u2010of\u2010flight mass spectrometry (UHPLC\u2013Q\u2010TOF\u2013MS\/MS). Finally, prototype constituents and related metabolites were analyzed after orally administerng 50EtLR to rats. As a result, a new compound, 6\u2010<jats:italic>O<\/jats:italic>\u2010[<jats:italic>\u03b2<\/jats:italic>\u2010<jats:sc>d<\/jats:sc>\u2010xylopyranosyl\u2010(1\u2009\u2192\u20096)\u2010<jats:italic>O<\/jats:italic>\u2010<jats:italic>\u03b2<\/jats:italic>\u2010<jats:sc>d<\/jats:sc>\u2010glucopyranosyl]\u20101,4,8\u2010trimethoxyxanthone (<jats:bold>6<\/jats:bold>) along with seven known compounds (<jats:bold>1\u20135<\/jats:bold>, <jats:bold>7<\/jats:bold> and <jats:bold>8<\/jats:bold>) were isolated from the 50EtLR, 92 components were either unambiguously or tentatively identified. Additionally, 34 prototype constituents and 112 metabolites in rat plasma along with 32 prototype constituents and 53 metabolites in rat liver were tentatively identified. Therefore, xanthones and flavonoids were the main chemical constituents of 50EtLR and sulfation and glucuronidation are the main enzyme\u2010induced metabolic pathways involved post\u2010administration.<\/jats:p>","DOI":"10.1002\/bmc.4651","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T05:44:31Z","timestamp":1563342271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Chemical fingerprint analysis and metabolic profiling of 50% ethanol fraction of <scp><i>Lomatogonium rotatum<\/i><\/scp> by ultra\u2010performance liquid chromatography\/quadrupole\u2013time of flight mass spectrometry"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4498-2734","authenticated-orcid":false,"given":"Lanjun","family":"Zhao","sequence":"first","affiliation":[{"name":"Jiangxi University of Traditional Chinese Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Ouyang","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donghui","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Innovative Drug and Efficient Energy\u2010Saving Pharmaceutical Equipment  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Hospital  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinlin","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangxi University of Traditional Chinese Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhifeng","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangxi University of Traditional Chinese Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Pan","sequence":"additional","affiliation":[{"name":"Jiangxi University of Traditional Chinese Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulin","family":"Feng","sequence":"additional","affiliation":[{"name":"National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herb Medicine  Nanchang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.37285\/ijpsn.2011.4.2.3"},{"key":"e_1_2_7_3_1","first-page":"789","article-title":"Study on anti\u2010hepatitis B virus effect of Lomatogonium rotatum in vitro","volume":"33","author":"Bai M. 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