{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:31:26Z","timestamp":1761611486679,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The combination of acebrophylline (ABP), levocetirizine (LCZ) and pranlukast (PRN) is used to treat allergic rhinitis, asthma, hay\u2010fever and other conditions where patients experience difficulty in breathing. This study was carried out with the aim of developing and validating a reverse\u2010phase high\u2010performance liquid chromatographic bioanalytical method to simultaneously quantitate ABP, LCZ and PRN in rat plasma. The objective also includes determination of the pharmacokinetic interaction of these three drugs after administration via the oral route after individual and combination treatment in rat. Optimum resolution between the analytes was observed with a C<jats:sub>18<\/jats:sub> Kinetex column (250 mm\u2009\u00d7\u20094.6\u2009mm\u2009\u00d7\u20095\u2009\u03bcm). The chromatography was performed in a gradient elution mode with a 1\u2009mL\/min flow rate. The calibration curves were linear over the concentration range of 100\u20131600\u2009ng\/mL. The intra\u2010 and inter\u2010day precision and accuracy were found to be within acceptable limits as specified in US Food and Drug Administration guideline for bioanalytical method validation. The analytes were stable on the bench\u2010top (8\u2009h), after three freeze\u2013thaw cycles, in the autosampler (8\u2009h) and as a dry extract (\u221280\u00b0C for 48\u2009h). The statistical results of the pharmacokinetic study in Sprague\u2013Dawley rats showed a significant change in pharmacokinetic parameters for PRN upon co\u2010administration of the three drugs.<\/jats:p>","DOI":"10.1002\/bmc.4672","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T09:28:57Z","timestamp":1565083737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Simultaneous bioanalysis and pharmacokinetic interaction study of acebrophylline, levocetirizine and pranlukast in Sprague\u2013Dawley rats"],"prefix":"10.1002","volume":"33","author":[{"given":"Priyanka","family":"Lohar","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Ahmedabad Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manish Kumar","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Ahmedabad Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit Kumar","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Ahmedabad Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeswari","family":"Rathod","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Ahmedabad Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6914-7057","authenticated-orcid":false,"given":"Pinaki","family":"Sengupta","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis National Institute of Pharmaceutical Education and Research  Ahmedabad Gujarat India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9]]},"reference":[{"issue":"6","key":"e_1_2_6_2_1","first-page":"381","article-title":"Long term treatment with montelukast and levocetirizine combination in persistent allergic rhinitis: Review of recent evidence","volume":"108","author":"Adsule S. 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