{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:34:41Z","timestamp":1786977281049,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T00:00:00Z","timestamp":1568073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A novel and robust two\u2010dimensional liquid chromatography with ultraviolet detection method (2D\u2010LC\u2013UV) was developed and validated for high\u2010throughput determination of the concentrations of valproic acid (VPA) in human plasma. This 2D\u2010LC system was composed of a first\u2010dimensional LC column, a second\u2010dimensional LC column and an intermediate transfer column. The sample was directly injected into the 2D\u2010LC system after an easy protein precipitation treatment. After online preconcentration and primary separation by the first\u2010dimensional column, the target was captured by an intermediate column and then transferred to second\u2010dimensional column for analysis. The system transferred the target through \u201ccentral cutting\u201d mode whereby the drug peak was not subject to interference from the matrix. The analysis cycle time was completed within 7.0\u2009min. Compared with other methods that have been developed, the analysis time was reduced and the operation was much easier without any derivatization. The calibration curve was linear over the 5.90\u2013188.94\u2009\u03bcg\/ml range for the VPA concentrations. The intra\u2010day and inter\u2010day precisions were &lt;5.6%. The recoveries were in the range from 95.2 to 98.0%. This method appears to be sensitive, precise, rapid and low\u2010cost for the quantification of VPA in serum sample.<\/jats:p>","DOI":"10.1002\/bmc.4695","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T09:18:15Z","timestamp":1567156695000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A novel and nonderivatization method for the determination of valproic acid in human serum by two\u2010dimensional liquid chromatography"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8115-6693","authenticated-orcid":false,"given":"Wei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Affiliated Guangji Hospital of Soochow University  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Guangji Hospital of Soochow University  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyong","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Guangji Hospital of Soochow University  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Second Xiangya Hospital of Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seizure.2007.12.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seizure.2011.10.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immbio.2008.01.004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yebeh.2005.04.010"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.05.022"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.9.2.285"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.01.086"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201101092"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-116492"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201500684"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.07.009"},{"issue":"3","key":"e_1_2_7_13_1","first-page":"126","article-title":"Evaluation of therapeutic drug monitoring of antiepileptic drugs","volume":"41","author":"Irshaid Y. M.","year":"2003","journal-title":"International Journal of Clinical Pharmacology and Therapeutics"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.15.162"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600202"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.08.014"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3904\/kjim.2009.24.1.1"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.06.086"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.08.002"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(04)00600-2"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-019-01859-2"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4440"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dmpk.2015.09.002"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.05.059"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.11.013"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.02.061"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.02.051"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199631020-00004"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmt167"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000256"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.566"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-019-01746-w"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4695","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4695","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.4695","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4695","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:49:21Z","timestamp":1760050161000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4695"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,10]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1002\/bmc.4695"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4695","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,10]]},"assertion":[{"value":"2019-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4695"}}