{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T10:41:32Z","timestamp":1773052892315,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A protein precipitation method for the determination of clobazam (CLB) and its major active metabolite <jats:italic>N<\/jats:italic>\u2010desmethylclobazam (<jats:italic>N\u2010<\/jats:italic>CLB) in human plasma by liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS) was established. CLB and <jats:italic>N\u2010<\/jats:italic>CLB were extracted from human plasma samples by protein precipitation with methanol. Analyte separation was done using a Phenomenex Kinetex\u2122 Biphenyl (50\u2009\u00d7\u20092.1\u2009mm, 1.7\u2009\u03bcm) column using isocratic elution with a mobile phase of 5\u2009m<jats:sc>m<\/jats:sc> ammonium formate with 0.01% ammonium hydroxide (40%) and methanol (60%) at a flow rate of 0.4\u2009mL\/min and an injection volume of 10\u2009\u03bcL. The detection was performed on a triple quadrupole mass spectrometer in multiple reaction monitoring mode to monitor the precursor\u2010to\u2010product ion transitions of <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 301.1\u2009\u2192\u2009259.0, 306.0\u2009\u2192\u2009263.9 for CLB and CLB\u2010D5 and 287.0\u2009\u2192\u2009245.0, 292.0\u2009\u2192\u2009250.0 for <jats:italic>N\u2010<\/jats:italic>CLB and <jats:italic>N\u2010<\/jats:italic>CLB\u2010D5 in positive electrospray ionization mode, respectively. The method was validated over a concentration range of 2.0\u2013750\u2009ng\/mL for CLB and 0.7\u2013200\u2009ng\/mL for <jats:italic>N\u2010<\/jats:italic>CLB on SCIEX Triple Quad 4500 MS System. Total run time was 5\u2009min. This method has been designed for bioequivalence study for formulations containing 20\u2009mg of CLB.<\/jats:p>","DOI":"10.1002\/bmc.4710","type":"journal-article","created":{"date-parts":[[2019,10,19]],"date-time":"2019-10-19T20:04:21Z","timestamp":1571515461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Protein precipitation method for determination of <scp>c<\/scp>lobazam and <scp><i>N<\/i><\/scp>\u2010desmethylclobazam in human plasma by <scp>LC\u2013MS\/MS<\/scp>"],"prefix":"10.1002","volume":"34","author":[{"given":"Astghik","family":"Mikayelyan","sequence":"first","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armine","family":"Aleksanyan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariam","family":"Sargsyan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arpine","family":"Gevorgyan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hasmik","family":"Zakaryan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armen","family":"Harutyunyan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lusine","family":"Zhamharyan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeghig","family":"Armoudjian","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8616-8821","authenticated-orcid":false,"given":"Tigran","family":"Margaryan","sequence":"additional","affiliation":[{"name":"DarmanTest Laboratories LLC  Yerevan Armenia"},{"name":"Scientific Research Center Yerevan State Medical University  Yerevan Armenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365519609090595"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.12017"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00440-0"},{"issue":"6","key":"e_1_2_5_5_1","first-page":"2369","article-title":"A rapid and sensitive LC\u2013MS\/MS assay for the determination of clobazam in human plasma using electro spray ionization technology","volume":"9","author":"Christian S.","year":"2018","journal-title":"International Journal of Pharmaceutical Sciences and Research"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.12.42"},{"key":"e_1_2_5_7_1","unstructured":"European Medicines Agency Guideline on bioanalytical method validation. 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