{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:54:06Z","timestamp":1761512046357,"version":"build-2065373602"},"reference-count":12,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,1,14]],"date-time":"2005-01-14T00:00:00Z","timestamp":1105660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, rapid, sensitive and speci\ufb01c liquid chromatography\u2013tandem mass spectrometry method was developed and validated for quanti\ufb01cation of mosapride (I), a novel and potent gastroprokinetic agent that enhances the upper gastrointestinal motility by stimulating 5\u2010HT<jats:sub>4<\/jats:sub> receptor. The analyte and internal standard, tamsulosin (II), were extracted by liquid\u2013liquid extraction with diethyl ether\u2013dichloromethane (70:30, v\/v) using a Glas\u2010Col Multi\u2010Pulse Vortexer. The chromatographic separation was performed on a reversed\u2010phase Waters symmetry C<jats:sub>18<\/jats:sub> column with a mobile phase of 0.03% formic acid\u2013acetonitrile (10:90, v\/v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The mass transitions <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 422.3 \u2192 198.3 and <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 409.1 \u2192 228.1 were used to measure I and II, respectively. The assay exhibited a linear dynamic range of 0.5\u2013100.0 ng\/mL for mosapride in human plasma. The lower limit of quantitation was 500 pg\/mL with a relative standard deviation of less than 15%. Acceptable precision and accuracy were obtained for concentrations over the standard curve ranges. A run time of 2.0 min for each sample made it possible to analyze a throughput of more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.477","type":"journal-article","created":{"date-parts":[[2005,1,14]],"date-time":"2005-01-14T12:01:20Z","timestamp":1105704080000},"page":"539-548","source":"Crossref","is-referenced-by-count":16,"title":["Validation and application of a high\u2010performance liquid chromatography\u2013tandem mass spectrometry assay for mosapride in human plasma"],"prefix":"10.1002","volume":"19","author":[{"given":"N. V. 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