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GHB was extracted from VAMS device by acetonitrile, after a re\u2010hydration step in a temperature\u2010controlled ultrasonic bath at 60\u00b0C for 10\u2009min. Chromatographic analysis was carried out on a Kinetex C<jats:sub>18<\/jats:sub> column using 0.1% formic acid in water and acetonitrile as binary gradient mobile phase (from 5 to 95% of acetonitrile from 1 to 2.5\u2009min) at a flow rate of 0.3\u2009ml\/min. The VAMS method was fully validated according to current guidelines with satisfactory results in terms of linearity, selectivity, precision, absolute recovery, matrix effect and stability. The linearity was determined from 0.5 to 200\u2009\u03bcg\/ml and the lower limit of quantitation was 0.5 \u03bcg\/ml. The novel VAMS\u2013UHPLC\u2013MS\/MS method was successfully compared with plasma\u2010based method in a GHB\u2010treated patient as a proof of concept.<\/jats:p>","DOI":"10.1002\/bmc.4781","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T03:54:36Z","timestamp":1576554876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development and validation of volumetric absorptive microsampling coupled with UHPLC\u2013MS\/MS for the analysis of gamma\u2010hydroxybutyric acid in human blood"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3311-1404","authenticated-orcid":false,"given":"Susan","family":"Mohamed","sequence":"first","affiliation":[{"name":"IRCCS\u2010Istituto delle Scienze Neurologiche di Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Riva","sequence":"additional","affiliation":[{"name":"IRCCS\u2010Istituto delle Scienze Neurologiche di Bologna  Bologna Italy"},{"name":"Department of Biomedical and Neuromotor Sciences University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Moresco","sequence":"additional","affiliation":[{"name":"IRCCS\u2010Istituto delle Scienze Neurologiche di Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Plazzi","sequence":"additional","affiliation":[{"name":"IRCCS\u2010Istituto delle Scienze Neurologiche di Bologna  Bologna Italy"},{"name":"Department of Biomedical and Neuromotor Sciences University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6399-9999","authenticated-orcid":false,"given":"Manuela","family":"Contin","sequence":"additional","affiliation":[{"name":"IRCCS\u2010Istituto delle Scienze Neurologiche di Bologna  Bologna Italy"},{"name":"Department of Biomedical and Neuromotor Sciences University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0091270002239707"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X13666141210215423"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/15563650.2018.1519194"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.01.022"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000643"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph13030290"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra044047"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.12.009"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60237-2"},{"key":"e_1_2_8_11_1","unstructured":"EMEA(2011).EMEA\/CHMP\/EWP\/192217\/2009. 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