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To evaluate the pharmacokinetics of ERAs, a rapid ultra\u2010performance liquid chromatography tandem mass spectrometry method was developed and validated to determine the concentration of ambrisentan, macitentan and sitaxentan in human plasma. Plasma samples were treated with methanol to induce protein precipitation. A chromatographic separation was performed on a C<jats:sub>18<\/jats:sub> column using a gradient of methanol\u2013water containing 0.1% formic acid and 0.013% ammonium acetate and a flow rate of 0.5\u2009ml\/min. Multiple reaction monitoring was used for quantification. This method was validated in a linear range of 20.28\u20132028\u2009\u03bcg\/l for ambrisentan, 4.052\u2013405.2\u2009\u03bcg\/l for macitentan and 205.4\u201310 270\u2009\u03bcg\/l for sitaxentan. The method was successfully validated according to US Food and Drug Administration guidelines to determine the concentrations of macitentan, ambrisentan and sitaxentan in human plasma. This method is now being used for study samples and clinical patient samples.<\/jats:p>","DOI":"10.1002\/bmc.4787","type":"journal-article","created":{"date-parts":[[2019,12,25]],"date-time":"2019-12-25T18:19:45Z","timestamp":1577297985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Simultaneous quantification of ambrisentan, macitentan and sitaxentan in human plasma using UPLC\u2013MS\/MS"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0692-6747","authenticated-orcid":false,"given":"Daan","family":"van de Velde","sequence":"first","affiliation":[{"name":"Department of Hospital Pharmacy Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0370-0614","authenticated-orcid":false,"given":"Soma","family":"Bahmany","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilie","family":"Hitzerd","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Division of Neonatology Erasmus University Medical Center  Rotterdam The Netherlands"},{"name":"Department of Internal Medicine, Division of Vascular Medicine and Pharmacology Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"van Domburg","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorie","family":"Versmissen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Vascular Medicine and Pharmacology Erasmus University Medical Center  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.H. 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