{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:56:36Z","timestamp":1790510196549,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,4,12]],"date-time":"2005-04-12T00:00:00Z","timestamp":1113264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Simple and reproducible HPLC methods for the determination of piperacillin and tazobactam have been developed and a complete stability study carried out. The method for piperacillin plasma samples consisted of protein precipitation with methanol using penicillin G as internal standard. No sample preparation was needed for ultrafiltrate samples. Tazobactam sample preparation involved protein precipitation with acetonitrile and the removal of lipids with dichloromethane. Piperacillin separation was performed on a \u00b5Bondapack\u2122 C<jats:sub>18<\/jats:sub> column (300 \u00d7 3.9, 10 \u00b5m) and tazobactam on a Novapack\u2122 C<jats:sub>18<\/jats:sub> column (150 \u00d7 3.9, 4 \u00b5m) with UV detection set at 229 and 225 nm, respectively. The mobile phase consisted of phosphate buffer\u2013acetonitrile, delivered at 1.5 mL[sol ]min. Calibration curves determination coefficients were \u22650.999 and response factors CV% &lt; 5%. Intra\u2010 and inter\u2010assay precision and accuracy of the quality control and limit of quantification were satisfactory. Plasma and ultrafiltrate samples were stable at \u221220 and \u221280\u00b0C for 2 months and after three freeze\u2013thaw cycles. In the chromatographic rack, tazobactam ultrafiltrate samples were stable for 24 h and plasma samples for 12 h, piperacillin ultrafiltrate samples for 8 h, but plasma samples for only 4 h. Storage of piperacillin samples at 4\u00b0C until analysis is recommended. Piperacillin was stable in the presence of tazobactam. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bmc.482","type":"journal-article","created":{"date-parts":[[2005,4,12]],"date-time":"2005-04-12T12:04:59Z","timestamp":1113307499000},"page":"570-578","source":"Crossref","is-referenced-by-count":22,"title":["Quantitation and stability of piperacillin and tazobactam in plasma and ultrafiltrate from patients undergoing continuous venovenous hemofiltration by HPLC"],"prefix":"10.1002","volume":"19","author":[{"given":"A.","family":"Arzuaga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Isla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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