{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:32:07Z","timestamp":1768285927475,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to demonstrate the altered metabolic infrastructure of pregnant women with methylenetetrahydrofolate reductase (MTHFR) polymorphisms at first trimester and during delivery. Eight singleton pregnant women with MTHFR polymorphisms were compared with 10 normal pregnant women. Maternal blood samples were obtained twice during their pregnancy period (between the 11th and 14th gestational weeks and during delivery). Metabolomic analysis was performed using GC\u2013MS. The GC\u2013MS based metabolomic profile helped identify 95 metabolites in the plasma samples. In the MTHFR group, the levels of 1\u2010monohexadecanoylglycerol, pyrophosphate, benzoin, and linoleic acid significantly decreased (<jats:italic>P<\/jats:italic> \u02c2 0.05 for all), whereas the levels of glyceric acid, <jats:sc>l<\/jats:sc>\u2010tryptophan, <jats:sc>l<\/jats:sc>\u2010alanine, <jats:sc>l<\/jats:sc>\u2010proline, norvaline, <jats:sc>l<\/jats:sc>\u2010threonine, and myo\u2010inositol significantly increased (<jats:italic>P<\/jats:italic> \u02c2 0.01 for the first two metabolites, <jats:italic>P<\/jats:italic> \u02c2 0.05 for the others) at 11\u201314 gestational weeks. Conversely, the levels of benzoin, 1\u2010monohexadecanoylglycerol, pyruvic acid, <jats:sc>l<\/jats:sc>\u2010proline, phosphoric acid, epsilon\u2010caprolactam, and pipecolic acid significantly decreased in the MTHFR group, whereas metabolites such as hexadecanoic acid and 2\u2010hydroxybutyric acid increased significantly in the study group during delivery. An impaired energy metabolism pathway, vitamin B complex disorders, tendency for metabolic acidosis (oxidative stress), and the need for cell\/tissue support seem prevalent in pregnancies with MTHFR polymorphisms.<\/jats:p>","DOI":"10.1002\/bmc.4842","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T09:45:32Z","timestamp":1586339132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Metabolic infrastructure of pregnant women with methylenetetrahydrofolate reductase polymorphisms: A metabolomic analysis"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-7628","authenticated-orcid":false,"given":"Tuba","family":"Recber","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0578-4230","authenticated-orcid":false,"given":"Gokcen","family":"Orgul","sequence":"additional","affiliation":[{"name":"Division of Perinatology, Medical Faculty Department of Obstetrics and Gynecology, Hacettepe University Hospital  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8877-2803","authenticated-orcid":false,"given":"Emine","family":"Ayd\u0131n","sequence":"additional","affiliation":[{"name":"Division of Perinatology, Medical Faculty Department of Obstetrics and Gynecology, Hacettepe University Hospital  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8209-8248","authenticated-orcid":false,"given":"Atakan","family":"Tanacan","sequence":"additional","affiliation":[{"name":"Division of Perinatology, Medical Faculty Department of Obstetrics and Gynecology, Hacettepe University Hospital  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7337-6215","authenticated-orcid":false,"given":"Emirhan","family":"Nemutlu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1322-1665","authenticated-orcid":false,"given":"Sedef","family":"K\u0131r","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6362-787X","authenticated-orcid":false,"given":"Mehmet Sinan","family":"Beksac","sequence":"additional","affiliation":[{"name":"Division of Perinatology, Medical Faculty Department of Obstetrics and Gynecology, Hacettepe University Hospital  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201601048"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2018.1465552"},{"key":"e_1_2_7_4_1","first-page":"1","article-title":"Metabolomic identification of placental alterations in fetal growth restriction","volume":"11","author":"Bahado\u2010Singh R. O.","year":"2020","journal-title":"The Journal of Maternal\u2010Fetal & Neonatal Medicine"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-007-0367-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-200403000-00005"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2003.017244"},{"key":"e_1_2_7_8_1","first-page":"105","article-title":"Metabolic and hormonal effects of myo\u2010inositol in women with polycystic ovary syndrome: a double\u2010blind trial","volume":"13","author":"Costantino D.","year":"2009","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2815439"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01141.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023811019023"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-03-0897"},{"key":"e_1_2_7_13_1","first-page":"1","article-title":"The prevalence of homozygous MTHFR polymorphism(s) in a Turkish university hospital population that necessitated MTHFR polymorphism investigation","volume":"15","author":"Gumruk F.","year":"2018","journal-title":"Electronic Journal of General Medicine"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470774366"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1992.00530330045014"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2004.06.003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000367656"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.02.011"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3557"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2012.09.008"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1042479"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.09.063"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2015.04.005"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2004.01.019"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.numecd.2009.04.018"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1610"},{"key":"e_1_2_7_27_1","first-page":"463","article-title":"Association between maternal MTHFR polymorphisms and nonsyndromic cleft lip with or without cleft palate in offspring, a meta\u2010analysis based on 15 case\u2010control studies","volume":"8","author":"Pan X.","year":"2015","journal-title":"International Journal of Fertility & Sterility"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7350"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/85.1.285S"},{"issue":"1","key":"e_1_2_7_30_1","first-page":"1","article-title":"Methylenetetrahydrofolate Reductase Enzyme Mutations and Relationship of Homocysteine Vitamin B12 and Folate Blood Levels","volume":"19","author":"\u015eahin T. G.","year":"2016","journal-title":"Gynecology Obstetrics & Reproductive Medicine"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1132"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.113670"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-017-3069-y"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mgene.2014.12.002"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/01443615.2013.838547"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/mgme.1998.2714"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-009-0269-0"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks374"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4842","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4842","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.4842","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4842","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T23:35:05Z","timestamp":1760052905000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,20]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/bmc.4842"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4842","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,20]]},"assertion":[{"value":"2020-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4842"}}