{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T12:56:56Z","timestamp":1770814616664,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study established a spectrum\u2013effect relationship method for screening and quantifying the analgesic and anti\u2010inflammatory active ingredients in Angelicae Pubescentis Radix (AP) by ultra\u2010high\u2010performance liquid chromatography\u2013quadrupole mass spectrometry detector analysis (UPLC\u2013QDA). First, the fingerprint of AP was established to determine the common peaks. Next, six batches of AP samples, with significant differences, were selected for evaluation of pharmacological activity. Subsequently, the spectrum\u2013effect relationship was used to screen the active ingredients. Finally, the screened ingredients were quantified using UPLC\u2013QDA. In total, 21 common peaks were identified and four effective compounds (bergapten, columbianetin acetate, osthole and isoimperatorin) were selected using the gray relational analysis and partial least squares regression analysis. Quantitative analysis showed that the content of the four effective compounds was the highest in a randomly selected batch, S7 (Hubei). To our knowledge, this is the first attempt that evaluated the quality and spectrum\u2013effect relationship of AP by quantitative analysis and chemometrics. This study identified the key pharmacologically active components of AP and thereby improved the quality evaluation system of AP. This method has broad application prospects for screening effective components and will be helpful in establishing more reliable, scientific and reasonable quality standards for AP and other traditional Chinese medicines.<\/jats:p>","DOI":"10.1002\/bmc.4910","type":"journal-article","created":{"date-parts":[[2020,5,30]],"date-time":"2020-05-30T14:59:34Z","timestamp":1590850774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A strategy for qualitative and quantitative profiling of Angelicae Pubescentis Radix and detection of its analgesic and anti\u2010inflammatory components by spectrum\u2013effect relationship and multivariate statistical analysis"],"prefix":"10.1002","volume":"34","author":[{"given":"Ajiao","family":"Hou","sequence":"first","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9607-1107","authenticated-orcid":false,"given":"Liu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaxu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Man","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingyou","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haixue","family":"Kuang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Environmental Monitoring Central Station of Heilongjiang Province  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuhong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine Guangdong Pharmaceutical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2722-3330","authenticated-orcid":false,"given":"Hai","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Chinese Materia Medica Heilongjiang University of Chinese Medicine, Ministry of Education  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201400289"},{"key":"e_1_2_10_3_1","first-page":"6","article-title":"Quantification and efficient discovery of quality control markers for Emilia prenanthoidea dc. by fingerprint\u2013efficacy relationship modelling","volume":"156","author":"Jiang Z. 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