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The proposed novel methodology included the generation of half\u2010DBS and quarter\u2010DBS discs after initial blood collection using the full\u2010DBS discs. Accordingly, blood collection via DBS was performed in male BALB\/c mice following intravenous and oral dosing of darolutamide; in male Sprague Dawley rats following intravenous and oral dosing of filgotinib. The ISR data generated from the full\u2010DBS disc, half\u2010DBS disc and quarter\u2010DBS disc were compared for the assessment of the proposed methodology. Quantification of darolutamide and filgotinib was accomplished using liquid chromatography\u2010electrospray ionization\/tandem mass spectrometry methods. Darolutamide and filgotinib ISR samples, which were collected and prepared using full\u2010, half\u2010 and quarter\u2010DBS discs, met the acceptance criteria for ISR analysis. In conclusion, this is the first report showing a viable tool for the performance of ISR on DBS cards. The use of quarter\u2010 or half\u2010DBS discs would aid in not only ISR but also in long\u2010term storage experiments of analytes because it would avoid the need for additional blood sampling in patients.<\/jats:p>","DOI":"10.1002\/bmc.4938","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T11:34:25Z","timestamp":1593689665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Novel methodology to perform incurred sample reanalysis on dried blood spot cards: Experimental data using darolutamide and filgotinib"],"prefix":"10.1002","volume":"34","author":[{"given":"Vinay","family":"Kiran","sequence":"first","affiliation":[{"name":"Drug Metabolism and Pharmacokinetics, Jubilant Biosys, Industrial Suburb, Yeshwanthpur  Bangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhishek","family":"Dixit","sequence":"additional","affiliation":[{"name":"Drug Metabolism and Pharmacokinetics, Jubilant Biosys, Industrial Suburb, Yeshwanthpur  Bangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhavesh Babulal","family":"Gabani","sequence":"additional","affiliation":[{"name":"Drug Metabolism and Pharmacokinetics, Jubilant Biosys, Industrial Suburb, Yeshwanthpur  Bangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5768-1332","authenticated-orcid":false,"given":"Nuggehally R.","family":"Srinivas","sequence":"additional","affiliation":[{"name":"Jubilant Generics  Noida Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1359-8999","authenticated-orcid":false,"given":"Ramesh","family":"Mullangi","sequence":"additional","affiliation":[{"name":"Drug Metabolism and Pharmacokinetics, Jubilant Biosys, Industrial Suburb, Yeshwanthpur  Bangalore Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0857-6591"},{"key":"e_1_2_6_3_1","first-page":"139","article-title":"Validated LC\u2010MS\/MS method for quantitation of a selective JAK1 inhibitor, filgotinib in rat plasma and its application to a pharmacokinetics study in rats","volume":"8","author":"Dixit A.","year":"2020","journal-title":"ADMET & DMPK"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199324060-00004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4737"},{"key":"e_1_2_6_6_1","unstructured":"Gilead. 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