{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:43:34Z","timestamp":1780393414436,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:00:00Z","timestamp":1595289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As a traditional Chinese medicine, <jats:italic>Marsdenia tenacissima<\/jats:italic> (Roxb.) Wight et Arn. plays an indispensable role in clinical practice owing to its specific efficacy in treating malignant tumors, leukocythemia, cystitis and asthma. This study aimed to establish a novel and scientific LC\u2013MS\/MS approach to simultaneously determine tenacissoside B, H, G and I, caffeic acid, cryptochlorogenic acid, chlorogenic acid and neochlorogenic acid from <jats:styled-content style=\"fixed-case\"><jats:italic>M. tenacissima<\/jats:italic><\/jats:styled-content> extract within the rat plasma samples. Digoxin was used as the internal reference. All determinations were carried out using the Eclipse Plus C<jats:sub>18<\/jats:sub> column, and water (containing 0.1% formic acid) was used as the mobile phase A, while acetonitrile was the mobile phase B for gradient elution. The UPLC methods were validated, including calibration curves, accuracy, precision, stability and recovery of the total eight analytes, in accordance with the requirements for biopharmaceutical analysis. Moreover, the proposed approach was also used in comprehensive pharmacokinetic research on those eight analytes in rats following <jats:styled-content style=\"fixed-case\"><jats:italic>M. tenacissima<\/jats:italic><\/jats:styled-content> extract gavage. According to the pharmacokinetic parameters, tenacissoside B, I, H and G are the long\u2010acting and primary bioactive constituents in <jats:styled-content style=\"fixed-case\"><jats:italic>M. tenacissima<\/jats:italic><\/jats:styled-content> extract, with long mean residence times and high concentrations. Our findings shed light on the absorption mechanism and provide significant information for the clinical application of <jats:styled-content style=\"fixed-case\"><jats:italic>M. tenacissima<\/jats:italic><\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/bmc.4946","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T05:56:15Z","timestamp":1594274175000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Simultaneous determination of eight bioactive components in <i>Marsdenia tenacissima<\/i> extract in rat plasma by LC\u2013MS\/MS and its application in a pharmacokinetic study"],"prefix":"10.1002","volume":"34","author":[{"given":"SiYu","family":"Li","sequence":"first","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen Han","family":"Pei","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine Macau University of Science and Technology  Macau China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7497-1457","authenticated-orcid":false,"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Liaoning University of Traditional Chinese Medicine  Dalian P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,21]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"6309","article-title":"Pharmacokinetic and bioavailability study of tenacissoside G in rat plasma by UPLC\u2013MS\/MS","volume":"9","author":"Bao S. 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