{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:58:53Z","timestamp":1789048733962,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T00:00:00Z","timestamp":1597276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hypertension is a major risk factor for atherosclerosis and ischemic heart disease. Most hypertensive patients need a combination of antihypertensive agents to achieve therapeutic goals. A rapid, sensitive, and selective liquid chromatography\u2010tandem mass spectrometric method was developed and validated for simultaneous determination of enalapril maleate (ENA) and its major metabolite enalaprilat (ENAT), nitrendipine (NIT) and its major metabolite dehydronitrendipine (DNIT), and hydrochlorothiazide (HCT) in human plasma using felodipine as an internal standard (IS). The drugs were extracted from plasma using one\u2010step protein precipitation. Chromatographic separation was performed on a Symmetry C<jats:sub>18<\/jats:sub> column, with water and acetonitrile (10:90, v\/v) as mobile phase. The detection was carried out using multiple reaction monitoring mode and coupled with electrospray ionization source. Multiple reaction monitoring transitions were <jats:italic>m\/z<\/jats:italic> 377.1\u2009\u2192\u2009234.1 for ENA, <jats:italic>m\/z<\/jats:italic> 349.2\u2009\u2192\u2009206.1 for ENAT, <jats:italic>m\/z<\/jats:italic> 361.2\u2009\u2192\u2009315.1 for NIT, <jats:italic>m\/z<\/jats:italic> 359\u2009\u2192\u2009331 for DNIT, <jats:italic>m\/z<\/jats:italic> 295.9\u2009\u2192\u2009205.1 for HCT, and <jats:italic>m\/z<\/jats:italic> 384.1\u2009\u2192\u2009338 for felodipine (IS). The method was linear over concentration ranges of 1\u2013200, 20\u2013500, 5\u2013200, 2\u2013100, and 5\u2013200\u2009ng\/mL for ENA, ENAT, NIT, DNIT, and HCT, respectively, with <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> \u2265\u20090.99. Method validation was performed according to U.S. Food and Drug Administration guidelines. The validated method showed good sensitivity and selectivity and could be applied for therapeutic drug monitoring and bioequivalence studies.<\/jats:p>","DOI":"10.1002\/bmc.4955","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T11:41:37Z","timestamp":1595590897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Validated LC\u2013MS\/MS method for the simultaneous determination of enalapril maleate, nitrendipine, hydrochlorothiazide, and their major metabolites in human plasma"],"prefix":"10.1002","volume":"34","author":[{"given":"Mohammad Abdul\u2010Azim","family":"Mohammad","sequence":"first","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0397-7952","authenticated-orcid":false,"given":"Marianne Alphonse","family":"Mahrouse","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enas Abdel Hakeem","family":"Amer","sequence":"additional","affiliation":[{"name":"National Organization for Drug Control and Research (NODCAR)  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouran Saleh","family":"Elharati","sequence":"additional","affiliation":[{"name":"National Organization for Drug Control and Research (NODCAR)  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,13]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Goodman and Gilman's the pharmacological basis of therapeutics","author":"Brunton L.","year":"2006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.104"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-011-2111-6"},{"issue":"3","key":"e_1_2_7_5_1","first-page":"47","article-title":"Liquid chromatography\u2013tandem mass spectrometry (LC\u2010MS) method for the assignment of enalapril and enalaprilat in human plasma","volume":"1","author":"Danafar H.","year":"2016","journal-title":"Pharmaceutical and Biomedical Research"},{"issue":"11","key":"e_1_2_7_6_1","first-page":"786","article-title":"Simultaneous HPLC determination of enalapril and hydrochlorothiazide in human plasma and its pharmacokinetic application","volume":"2","author":"Foda N. 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