{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:36:11Z","timestamp":1789875371259,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1C1B5015865"],"award-info":[{"award-number":["2017R1C1B5015865"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cefpiramide is frequently used to treat biliary infections. However, no bioanalytical method has been validated to quantitate cefpiramide in human samples, particularly in bile. Therefore, this study was conducted to develop a simple, selective and validated high\u2010performance liquid chromatographic method to determine cefpiramide in human plasma and bile. A protein precipitation procedure was used to extract cefpiramide and cefoperazone (internal standard, IS) from 200\u2009\u03bcl of plasma and bile. Utilizing a Capcell Pak C<jats:sub>18<\/jats:sub> column (4.6\u2009\u00d7\u2009250\u2009mm), cefpiramide and IS were separated using the timed\u2010gradient mobile phase consisting of 0.1 <jats:sc>m<\/jats:sc> sodium acetate (pH\u20095.2) and acetonitrile at a flow rate of 1\u2009ml\/min with photodiode array detector (wavelength set at 273\u2009nm). The calibration curves showed linearity at concentrations ranging from 1 to 150\u2009\u03bcg\/ml in both plasma and bile (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009&gt;\u20090.999). The within\u2010 and between\u2010run coefficients of variation (CVs) for plasma samples were 0.570\u20134.43 and 1.10\u20132.76%, respectively; for bile samples, the within\u2010 and between\u2010day precision (CV) was 0.814\u20136.34 and 2.05\u20134.00%, respectively. Our newly developed bioanalytical method was successfully employed to quantify cefpiramide concentrations in both plasma and bile at multiple time points in patients with acute cholangitis.<\/jats:p>","DOI":"10.1002\/bmc.4957","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T12:40:38Z","timestamp":1595594438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Quantitation of plasma and biliary cefpiramide concentrations in human samples using high\u2010performance liquid chromatography"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5086-7073","authenticated-orcid":false,"given":"Jimin","family":"Yoon","sequence":"first","affiliation":[{"name":"Department of Pharmacy College of Pharmacy Kyung Hee University  Seoul South Korea"},{"name":"Department of Pharmacy Kyung Hee University Hospital at Gangdong  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won Gun","family":"Kwack","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy and Critical Care Medicine Kyung Hee University Hospital  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang\u2010Seob","family":"Shim","sequence":"additional","affiliation":[{"name":"Kyung Hee Drug Analysis Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Kyu","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Dongguk University Ilsan Hospital  Goyang Gyeonggi\u2010do South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Kee","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Dongguk University Ilsan Hospital  Goyang Gyeonggi\u2010do South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namyi","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Dongguk University Ilsan Hospital  Goyang Gyeonggi\u2010do South Korea"},{"name":"Department of Clinical Pharmacology and Therapeutics Dongguk University Clinical Trial Center  Goyang Gyeonggi\u2010do South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Yoon","family":"Cho","sequence":"additional","affiliation":[{"name":"Kyung Hee Drug Analysis Center  Seoul South Korea"},{"name":"Department of Pharmaceutical Biochemistry, College of Pharmacy Kyung Hee University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung\u2010Tae","family":"Lee","sequence":"additional","affiliation":[{"name":"Kyung Hee Drug Analysis Center  Seoul South Korea"},{"name":"Department of Pharmaceutical Biochemistry, College of Pharmacy Kyung Hee University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9200-2799","authenticated-orcid":false,"given":"Eun Kyoung","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Pharmacy College of Pharmacy Kyung Hee University  Seoul South Korea"},{"name":"Department of Pharmacy Kyung Hee University Hospital at Gangdong  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-014-3477-y"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinthera.2012.01.023"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/10.3.207"},{"key":"e_1_2_8_5_1","first-page":"418","article-title":"Cefpiramide, a new cephalosporin with high hepatic elimination; Experimental evaluation of its biliary passage and disposition in the liver","volume":"37","author":"Brogard J. M.","year":"1989","journal-title":"Pathologie\u2010Biologie"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.9.1360"},{"key":"e_1_2_8_7_1","first-page":"519","article-title":"Experimental and clinical evaluation of the biliary pharmacokinetic profile of cefpiramide, a new cephalosporin with high hepatic elimination","volume":"14","author":"Brogard J. M.","year":"1988","journal-title":"Drugs under Experimental and Clinical Research"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5675-y"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.31.10.1585"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80144-5"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1994.tb04278.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.1991.27"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80305-5"},{"key":"e_1_2_8_14_1","unstructured":"Food and Drug Administration. (2018).Bioanalytical method validation guidance for industry. US Department of Health and Human Services. Retrieved fromhttps:\/\/www.fda.gov\/media\/70858\/download"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhbp.518"},{"key":"e_1_2_8_16_1","unstructured":"International Council for Harmonization. (2019).ICH Guideline M10 on Bioanalytical Method Validation. Retrieved fromhttps:\/\/www.ema.europa.eu\/en\/documents\/scientific-guideline\/draft-ich-guideline-m10-bioanalytical-method-validation-step-2b_en.pdf"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb1978.11.839"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00515-19"},{"key":"e_1_2_8_19_1","unstructured":"Ministry of Food and Drug Safety. (2013).Guideline on bioanalytical method validation. Retrieved fromhttps:\/\/www.mfds.go.kr\/brd\/m_210\/down.do?brd_id=data0010&seq=13054&data_tp=A&file_seq=1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.36.1622"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1532"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.121910"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11604-019-00893-5"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbmc.4957","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4957","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.4957","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4957","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:09:20Z","timestamp":1760036960000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,4]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1002\/bmc.4957"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4957","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,4]]},"assertion":[{"value":"2020-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4957"}}