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Hence in the present work arginase inhibitor activity is studied from <jats:styled-content style=\"fixed-case\"><jats:italic>Ficus religiosa<\/jats:italic><\/jats:styled-content> leaves. A fine powder of <jats:styled-content style=\"fixed-case\"><jats:italic>F. religiosa<\/jats:italic><\/jats:styled-content> leaves was serially extracted in various solvents, viz. hexane, chloroform, ethyl acetate and methanol. Out of those four solvent extracts, the one showing highest arginase inhibitor activity was loaded onto the column for further fractionation. Among the collected fractions, the one showing the highest activity was subjected to identification of metabolites by using LC\u2013HRMS. Total compounds including acipimox, edoxudine, levulinic acid, hydroxyhydroquinone, ramiprilglucuronide, berberine, antimycin A, swietenine and some short peptides were identified from the fraction showing the highest arginase inhibitory activity. Identification of these metabolites from <jats:styled-content style=\"fixed-case\"><jats:italic>F. religiosa<\/jats:italic><\/jats:styled-content> and their biological importance may help to promote its use as medicinal plant. Further purification and characterization of therapeutically novel molecules will be the subject of future work.<\/jats:p>","DOI":"10.1002\/bmc.4966","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T07:57:25Z","timestamp":1597391845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolite profiling of arginase inhibitor activity guided fraction of <scp><i>Ficus religiosa<\/i><\/scp> leaves by LC\u2013HRMS"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4184-8991","authenticated-orcid":false,"given":"Ayesha","family":"Shaikh","sequence":"first","affiliation":[{"name":"Department of Biochemistry Dr Babasaheb Ambedkar Marathwada University  Aurangabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4567-9662","authenticated-orcid":false,"given":"Satishkumar","family":"Tekale","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Dr Babasaheb Ambedkar Marathwada University  Aurangabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5889-6220","authenticated-orcid":false,"given":"Sandip","family":"Wagh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Dr Babasaheb Ambedkar Marathwada University  Aurangabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1839-9238","authenticated-orcid":false,"given":"Manohar","family":"Padul","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Dr Babasaheb Ambedkar Marathwada University  Aurangabad India"},{"name":"Department of Biochemistry, The Institute of Science Dr Homi Bhabha State University  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21567"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0840"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.11.3148"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00194.2003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2013.03.009"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/S0026-895X(25)10969-3","article-title":"Mechanism of action of 5\u2010(2\u2010chloroethyl)\u20102\u2032\u2010deoxyuridine, a selective inhibitor of herpes simplex virus replication","volume":"37","author":"Clercq E.","year":"1990","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.02.008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.241836"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2013.09.003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13410-012-0109-8"},{"key":"e_1_2_9_12_1","first-page":"1395","article-title":"In vitro cytotoxic potential of Swietenia macrophylla King seeds against human carcinoma cell lines","volume":"5","author":"Goh B. 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