{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T01:18:32Z","timestamp":1782004712275,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T00:00:00Z","timestamp":1599350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006441","name":"Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006441","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biomedical Chromatography"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nimodipine is a dihydropyridine calcium channel blocker that exhibits higher selectivity toward cerebral blood vessels compared with other members of the same class. It has been shown to improve outcomes and prevent delayed cerebral ischemia in the setting of aneurysmal subarachnoid hemorrhage, a life\u2010threatening brain bleed. Nimodipine is a chiral compound and it is marketed as a racemic mixture of (+)<jats:italic>\u2010R<\/jats:italic> and (\u2212)\u2010<jats:italic>S<\/jats:italic> enantiomers. (\u2212)\u2010<jats:italic>S<\/jats:italic>\u2010Nimodipine is approximately twice as potent a vasorelaxant as the racemic mixture and is more rapidly eliminated than the (+)<jats:italic>\u2010R<\/jats:italic> counterpart following oral dosing. Few analytical procedures have been reported to determine nimodipine enantiomers in biological samples; however, the reported methods were time\u2010consuming, involved multistep extraction procedures and required large sample volumes. Herein, we present an LC\u2013MS\/MS method for quantifying nimodipine enantiomers in human plasma using a small sample volume (0.3\u2009ml) and a single liquid\u2013liquid extraction step. The peak area ratios were linear over the tested concentration ranges (1.5\u201375\u2009ng\/ml) with <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009&gt;\u20090.99. The intraday CV and percentage error were within \u00b114% while the interday values were within \u00b113%, making this analytical method feasible for research purposes and pharmacokinetic studies.<\/jats:p>","DOI":"10.1002\/bmc.4971","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T15:18:04Z","timestamp":1598368684000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Enantioselective assay of nimodipine in human plasma using liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"35","author":[{"given":"Fadumo Ahmed","family":"Isse","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyson","family":"Le","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5517-2622","authenticated-orcid":false,"given":"Sherif Hanafy","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy and Pharmaceutical Sciences University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303173081103"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-82502010000400008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600820305"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)83318-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.06.012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00201-W"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1988.68.4.0505"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01456369"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.298.6674.636"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2007.10.035"},{"issue":"1","key":"e_1_2_7_12_1","first-page":"338","article-title":"The effect of nimodipine, its optical isomers and metabolites on isolated vascular smooth muscle","volume":"32","author":"Towart R.","year":"1982","journal-title":"Arzneimittel\u2010Forschung"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1520-636X(2000)12:9<660::AID-CHIR3>3.0.CO;2-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/48.2.81"}],"container-title":["Biomedical Chromatography"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4971","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bmc.4971","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bmc.4971","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:16:44Z","timestamp":1760055404000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.4971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,6]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/bmc.4971"],"URL":"https:\/\/doi.org\/10.1002\/bmc.4971","archive":["Portico"],"relation":{},"ISSN":["0269-3879","1099-0801"],"issn-type":[{"value":"0269-3879","type":"print"},{"value":"1099-0801","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,6]]},"assertion":[{"value":"2020-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4971"}}